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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment

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Author(s):
Marcela Paola Castro ARIAS [1] ; Amanda Garcia Alves MALIZA [2] ; Raquel Zanin MIDENA [3] ; Márcia Sirlene Zardin GRAEFF [4] ; Marco Antonio Húngaro DUARTE [5] ; Flaviana Bombarda de ANDRADE [6]
Total Authors: 6
Affiliation:
[1] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Dentística, Endodontia e Materiais Odontológicos - Brasil
[2] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Dentística, Endodontia e Materiais Odontológicos - Brasil
[3] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Dentística, Endodontia e Materiais Odontológicos - Brasil
[4] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Centro Integrado de Pesquisa - Brasil
[5] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Dentística, Endodontia e Materiais Odontológicos - Brasil
[6] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Dentística, Endodontia e Materiais Odontológicos - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Journal of Applied Oral Science; v. 24, n. 6, p. 575-581, 2016-12-00.
Abstract

ABSTRACT Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p<0.05). Conclusion The ultrasonic agitation of CH paste increased its antimicrobial action and was responsible for intradentinal penetration with the fulfilment of the tubules. (AU)

FAPESP's process: 10/20186-3 - Adherence factors and biofilms generated by root canal microorganisms
Grantee:Flaviana Bombarda de Andrade
Support Opportunities: Regular Research Grants