Adherence factors and biofilms generated by root canal microorganisms

• Grant number: 10/20186-3
• Support Opportunities: Regular Research Grants
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Flaviana Bombarda de Andrade
• Grantee: Flaviana Bombarda de Andrade
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated researchers: Marco Antonio Hungaro Duarte

Abstract

The endodontic microbiota is mainly anaerobic and proteolitic, due to this site conditions and disposable nutrients. Factors of adherence can promote the microbial installation at the root canal walls, forming biofilms, and also proliferation inside the dentinal tubules. The biofilm microorganisms change genetic material, increasing their resistance. Because of this, it is almost impossible a microbial clinical isolate having the genes totally equal to the pattern strains of species of culture collections, like ATCC (American Type Culture Collection). The aim of this study is investigate three species of common microorganisms of necrotic root canals, comparing five clinical isolates to the respective ATCC strain, evaluating some characteristics of planctonic and biofilm strains. The investigated anaerobic species will be Fusobacterium nucleatum and Bifidobacterium dentium, besides the facultative Enterococcus faecalis. The clinical isolates will be cultivated in culture media, isolated and identified by biochemical commercial tests (System Api, Bio-Meriéux, França). The static biofilms will be produced in celluloses' membrane, characterized by scanning electron microscopy (SEM), measured their volume by confocal microscopy and by counting of colonies at the microbial culture (colony forming units - CFU). Each species has specifics genes of adherence, which is an advantageous factor. The expression of these specific genes will be evaluated by technique of Real-time PCR, its major or minor production by planctonic bacteria or biofilm, from ATCC strain or clinical isolate. The accuracy and knowledge of the root canals microorganisms' behavior can promote better study models of antimicrobial effect of substances in the future, besides better knowledge of virulence of microorganisms. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARICEL ROSARIO CARDENAS CUELLAR; EVELYN GIULIANA VELÁSQUEZ-ESPEDILLA; VICTOR FELIZ PEDRINHA; RODRIGO RICCI VIVAN; MARCO ANTONIO HUNGARO DUARTE; FLAVIANA BOMBARDA DE ANDRADE. Can kinematics, file diameter, and PUI influence the intracanal decontamination and apical bacterial extrusion?. Brazilian Oral Research, v. 35, 2020-11-13. (10/20186-3)

RODRIGUES, C. T.; DE ANDRADE, F. B.; DE VASCONCELOS, L. R. S. M.; MIDENA, R. Z.; PEREIRA, T. C.; KUGA, M. C.; DUARTE, M. A. H.; BERNARDINELI, N.. Antibacterial properties of silver nanoparticles as a root canal irrigant against Enterococcus faecalis biofilm and infected dentinal tubules. International Endodontic Journal, v. 51, n. 8, p. 901-911, AUG 2018. (10/20186-3)

MARCELA PAOLA CASTRO ARIAS; AMANDA GARCIA ALVES MALIZA; RAQUEL ZANIN MIDENA; MÁRCIA SIRLENE ZARDIN GRAEFF; MARCO ANTONIO HÚNGARO DUARTE; FLAVIANA BOMBARDA DE ANDRADE. Effect of ultrasonic streaming on infra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment. Journal of Applied Oral Science, v. 24, n. 6, p. 575-581, Dez. 2016. (10/20186-3)

MARCELA PAOLA CASTRO ARIAS; AMANDA GARCIA ALVES MALIZA; RAQUEL ZANIN MIDENA; MÁRCIA SIRLENE ZARDIN GRAEFF; MARCO ANTONIO HÚNGARO DUARTE; FLAVIANA BOMBARDA DE ANDRADE. Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment. Journal of Applied Oral Science, v. 24, n. 6, p. 575-581, 2016-12-00. (10/20186-3)

PEREIRA, THAIS CRISTINA; DA SILVA MUNHOZ VASCONCELOS, LAYLA REGINNA; ZARDIN GRAEFF, MARCIA SIRLENE; MARCUCCI RIBEIRO, MARIA CRISTINA; HUNGARO DUARTE, MARCO ANTONIO; DE ANDRADE, FLAVIANA BOMBARDA. Intratubular decontamination ability and physicochemical properties of calcium hydroxide pastes. CLINICAL ORAL INVESTIGATIONS, v. 23, n. 3, p. 1253-1262, MAR 2019. (13/26120-2, 10/20186-3)

SILVA MUNHOZ DE VASCONCELOS, LAYLA REGINNA; MIDENA, RAQUEL ZANIN; MINOTTI, PALOMA GAGLIARDI; PEREIRA, THAIS CRISTINA; HUNGARO DUARTE, MARCO ANTONIO; DE ANDRADE, FLAVIANA BOMBARDA. Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems. Journal of Applied Oral Science, v. 25, n. 5, p. 477-482, SEP-OCT 2017. (10/20186-3, 11/18479-5)

PEDRINHA, VICTOR FELIZ; CARDENAS CUELLAR, MARICEL ROSARIO; VELASQUEZ-ESPEDILLA, EVELYN GIULIANA; HUNGARO DUARTE, MARCO ANTONIO; DE ANDRADE, FLAVIANA BOMBARDA; RODRIGUES, PATRICIA DE ALMEIDA. Impact of irrigation protocols with some chelators and mechanical agitation on intratubular decontamination. Brazilian Oral Research, v. 35, 2021. (10/20186-3)

DE ANDRADE, FLAVIANA BOMBARDA; CASTRO ARIAS, MARCELA PAOLA; ALVES MALIZA, AMANDA GARCIA; HUNGARO DUARTE, MARCO ANTONIO; ZARDIN GRAEFF, MARCIA SIRLENE; AMOROSO-SILVA, PABLO ANDRES; MIDENA, RAQUEL ZANIN; DE MORAES, IVALDO GOMES. A new improved protocol for in vitro intratubular dentinal bacterial contamination for antimicrobial endodontic tests: standardization and validation by confocal laser scanning microscopy. Journal of Applied Oral Science, v. 23, n. 6, p. 591-598, NOV-DEC 2015. (10/20186-3)

DE ANDRADE, FLAVIANA BOMBARDA; DA SILVA MUNHOZ VASCONCELOS, LAYLA REGINNA; PEREIRA, THAIS CRISTINA; GARCIA, ROBERTO BRANDAO; BRAMANTE, CLOVIS MONTEIRO; DUARTE, MARCO ANTONIO HUNGARO. Ultrasonic agitation reduces the time of calcium hydroxide antimicrobial effect and enhances its penetrability. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 32, n. 12, DEC 2021. (10/20186-3)

CARDENAS CUELLAR, MARICEL ROSARIO; VELASQUEZ-ESPEDILLA, EVELYN GIULIANA; PEDRINHA, VICTOR FELIZ; VIVAN, RODRIGO RICCI; HUNGARO DUARTE, MARCO ANTONIO; DE ANDRADE, FLAVIANA BOMBARDA. Can kinematics, file diameter, and PUI influence the intracanal decontamination and apical bacterial extrusion?. BRAZILIAN ORAL RESEARCH, v. 35, p. 10-pg., 2021-01-01. (10/20186-3)

SILVA MUNHOZ DE VASCONCELOS, LAYLA REGINNA; MIDENA, RAQUEL ZANIN; MINOTTI, PALOMA GAGLIARDI; PEREIRA, THAIS CRISTINA; HUNGARO DUARTE, MARCO ANTONIO; DE ANDRADE, FLAVIANA BOMBARDA. Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems. Journal of Applied Oral Science, v. 25, n. 5, p. 6-pg., 2017-09-01. (10/20186-3, 11/18479-5)