Proteome profiling reveals insights into secondary metabolism in Maytenus ilicifolia (Celastraceae) cell cultures producing quinonemethide triterpenes

Author(s)

Paz, Tiago Antunes ; dos Santos, Vania A. F. F. M. ; Inacio, Marielle Cascaes ; Dias, Nathalia Batista ; Palma, Mario Sergio ; Soares Pereira, Ana Maria ; Furlan, Maysa

• Document type: Journal article
• Source: PLANT CELL TISSUE AND ORGAN CULTURE; v. 130, n. 2, p. 405-416, AUG 2017.
• Web of Science Citations: 2

Abstract

Plants produce a wide variety of secondary metabolites (SMs) of pharmacological interest that, due to their high structural complexity, are difficult to obtain from chemical synthesis. Knowledge of the enzymatic process involved in the biosynthesis of these SMs may contribute to the development of new strategies for obtaining them from plant tissues. Maytenus ilicifolia contains three main classes of bioactive compounds: sesquiterpene pyridine alkaloids, flavonoids and, primarily, quinonemethide triterpenes, which are well known for their high and broad antitumor activity. Thus, to determine the enzymatic composition in the secondary metabolism of M. ilicifolia, we carried out proteomic profiling of M. ilicifolia cell cultures with and without increased concentrations of maytenin and 22 beta-hydroxy-maytenin by elicitation with methyl jasmonate for 48 h. For this purpose, the analysis of soluble proteins was performed via LCMS/MS experiments using a shotgun strategy and compared to specific databases, resulting in 1319 identifications in the two types of cells. Many enzymes involved in secondary metabolism were detected, including cytochrome P450-dependent monooxygenases, which potentially catalyze the final steps in the quinonemethide triterpene biosynthesis. In conclusion, we report for the first time a proteomic study of a cell culture system that is an effective elicitor of quinonemethide triterpene production. (AU)

• FAPESP's process: 14/19362-2 - Application of biotechnology to evaluate plant-microorganism interaction during production of secondary metabolites in Peritassa campestris and its endophytes
• Grantee: Marielle Cascaes Inácio
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/01544-9 - Proteomic analysis focused on the enzyme P450 oxidoreductase in roots of young plants and adult Maytenus ilicifolia (Celastraceae)
• Grantee: Vânia Aparecida de Freitas Formenton Macedo dos Santos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC