| Full text | |
| Author(s): |
Ribeiro, Vanessa R.
;
Romao-Veiga, Mariana
;
Romagnoli, Graziela G.
;
Matias, Mariana L.
;
Nunes, Priscila R.
;
Borges, Vera Therezinha M.
;
Peracoli, Jose C.
;
Peracoli, Maria Terezinha S.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | IMMUNOLOGY; v. 152, n. 1, p. 163-173, SEP 2017. |
| Web of Science Citations: | 17 |
| Abstract | |
Pre-eclampsia (PE) is an obstetric pathology characterized by abnormal activation of the innate and adaptive immune systems dependent on the imbalance of T helper subsets. The present study aimed to evaluate the gene and protein expression of T helper type 1 (Th1)/Th2/Th17/regulatory T (Treg) cell transcription factors in peripheral blood lymphocytes from pregnant women with PE employing quantitative RT-PCR and flow cytometry techniques, as well as the cytokine profile produced by these CD4(+) T-cell subsets in the plasma of pregnant women with PE, classified as early-onset PE (n = 20), late-onset PE (n = 20) and normotensive pregnant women (n = 20). Results showed a higher percentage of CD4(+) T cells expressing the RORc transcription factor (Th17) and a lower percentage of cells expressing FoxP3 (Treg) in women with early-onset PE compared with late-onset PE and normotensive groups. A lower gene expression of GATA-3 transcription factor was detected in cells of women with early-onset PE compared with the late-onset PE group. Endogenous plasma levels of interleukin-6 (IL-6), IL-17 and tumour necrosis factor- were significantly higher in the early-onset PE group than in the late-onset PE and normotensive groups, whereas IL-4 (Th2 profile) and IL-22 (Th17 profile), were not significantly different between the studied groups. The endogenous levels of transforming growth factor- and IL-10 were significantly lower in the pre-eclamptic than in the normotensive groups of the same gestational age, with a significant difference between early- and late-onset PE. The results show that in women with PE there is an imbalance between inflammatory and anti-inflammatory profiles in CD4(+) T-cell subsets, with polarization to Th17 profiles in the early-onset PE, considered as the severe form of PE. (AU) | |
| FAPESP's process: | 12/24697-8 - INFLAMMASOME AND AUTOPHAGY INVOLVEMENT IN THE PATHOPHYSIOLOGY OF PREECLAMPSIA |
| Grantee: | Maria Terezinha Serrão Peraçoli |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/25124-7 - ASSOCIATION BETWEEN CYTOKINE PROFILE AND TRANSCRIPTION FACTORS PRODUCED BY T CELLS SUBSETS IN EARLY- AND LATE- ONSET PREECLAMPSIA |
| Grantee: | Vanessa Rocha Ribeiro Vasques |
| Support Opportunities: | Scholarships in Brazil - Master |