Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Association between cytokine profile and transcription factors produced by T-cell subsets in early- and late-onset pre-eclampsia

Full text
Ribeiro, Vanessa R. ; Romao-Veiga, Mariana ; Romagnoli, Graziela G. ; Matias, Mariana L. ; Nunes, Priscila R. ; Borges, Vera Therezinha M. ; Peracoli, Jose C. ; Peracoli, Maria Terezinha S.
Total Authors: 8
Document type: Journal article
Source: IMMUNOLOGY; v. 152, n. 1, p. 163-173, SEP 2017.
Web of Science Citations: 17

Pre-eclampsia (PE) is an obstetric pathology characterized by abnormal activation of the innate and adaptive immune systems dependent on the imbalance of T helper subsets. The present study aimed to evaluate the gene and protein expression of T helper type 1 (Th1)/Th2/Th17/regulatory T (Treg) cell transcription factors in peripheral blood lymphocytes from pregnant women with PE employing quantitative RT-PCR and flow cytometry techniques, as well as the cytokine profile produced by these CD4(+) T-cell subsets in the plasma of pregnant women with PE, classified as early-onset PE (n = 20), late-onset PE (n = 20) and normotensive pregnant women (n = 20). Results showed a higher percentage of CD4(+) T cells expressing the RORc transcription factor (Th17) and a lower percentage of cells expressing FoxP3 (Treg) in women with early-onset PE compared with late-onset PE and normotensive groups. A lower gene expression of GATA-3 transcription factor was detected in cells of women with early-onset PE compared with the late-onset PE group. Endogenous plasma levels of interleukin-6 (IL-6), IL-17 and tumour necrosis factor- were significantly higher in the early-onset PE group than in the late-onset PE and normotensive groups, whereas IL-4 (Th2 profile) and IL-22 (Th17 profile), were not significantly different between the studied groups. The endogenous levels of transforming growth factor- and IL-10 were significantly lower in the pre-eclamptic than in the normotensive groups of the same gestational age, with a significant difference between early- and late-onset PE. The results show that in women with PE there is an imbalance between inflammatory and anti-inflammatory profiles in CD4(+) T-cell subsets, with polarization to Th17 profiles in the early-onset PE, considered as the severe form of PE. (AU)

FAPESP's process: 12/24697-8 - Inflammasome and autophagy involvement in the pathophysiology of preeclampsia
Grantee:Maria Terezinha Serrão Peraçoli
Support Opportunities: Regular Research Grants
FAPESP's process: 14/25124-7 - Association between cytokine profile and transcription factors produced by T cells subsets in early- and late- onset preeclampsia
Grantee:Vanessa Rocha Ribeiro Vasques
Support Opportunities: Scholarships in Brazil - Master