Sperm ultrastructure of shrimps from the family Penaeidae (Crustacea: Dendrobranchiata) in a phylogenetic context

Author(s)

Camargo, Tavani Rocha ; Rossi, Natalia ; Castilho, Antonio L. ; Costa, Rogerio C. ; Mantelatto, Fernando L. ; Zara, Fernando Jose

• Document type: Journal article
• Source: ARTHROPOD STRUCTURE & DEVELOPMENT; v. 46, n. 4, p. 588-600, JUL 2017.
• Web of Science Citations: 7

Abstract

We describe the sperm ultrastructure of six penaeid species, including at least one member of each tribe (Penaeini, Parapenaeini and Trachypenaeini). Fragments of the vas deferens of the Penaeidae Farfantepenaeus brasiliensis, Farfantepenaeus paulensis, Litopenaeus schmitti, Parapenaeus americanus, Rimapenaeus constrictus and Xiphopenaeus kroyeri were fixed and processed according to the routine for transmission electron microscopy. The morphological results were contextualized in an evolutionary perspective using molecular markers for the phylogenetic reconstruction of this group. A phylogram was proposed by Bayesian inference based on 1007 bp of 33 sequences of the combined genes (16S rDNA and COI mtDNA) from 27 dendrobranchiate specimens. Our findings show that morphological differences in the sperm ultrastructures of members among the tribes of Penaeidae can be used as a baseline to understand their evolutionary relationships. Individuals from the Penaeini tribe show plesiomorphic characteristics in the sperm ultrastructure compared to the Trachypenaeini tribe from which they were derived, such as shrimp from family Sicyoniidae. The morphological complexity of the sperm of the different penaeid members corroborated with the genetic phylogeny, which showed different Glades for each tribe and the close relationship with Sicyoniidae. The sperm features of the selected species studied here reflected their evolutionary history. These features confirm the previous phylogenetic hypothesis and question the monophyly of Penaeidae, which should be verified in the future with a more complete set of representative members of each tribe. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 16/11182-0 - Bioprospecting of antioxidant compounds in the by-catch of demersal fishing and use of these biomolecules as nutraceuticals in aquaculture
• Grantee: Tavani Rocha Camargo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/50188-8 - Decapod crustaceans: multi-disciplinary characterization of the sea biodiversity of the State of São Paulo (taxonomy, spermiotaxonomy, molecular biology and population dynamics)
• Grantee: Fernando Luis Medina Mantelatto
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants