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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hydrogen peroxide induces cell proliferation and apoptosis in pulp of rats after dental bleaching in vivo

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Author(s):
Benetti, Francine ; Gomes-Filho, Joao Eduardo ; Ferreira, Luciana Louzada ; Ervolino, Edilson ; Fraga Briso, Andre Luiz ; Sivieri-Araujo, Gustavo ; Dezan-Junior, Eloi ; Angelo Cintra, Luciano Tavares
Total Authors: 8
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 81, p. 103-109, SEP 2017.
Web of Science Citations: 17
Abstract

Objective: This study provides an in vivo evaluation of the inflammatory response, levels of cell proliferation and apoptosis, and the presence of necrosis after dental bleaching with two concentrations of hydrogen peroxide (H2O2). Design: Wistar rats were divided into Control (placebo gel), BLUE (20% H2O2, 1 x 50 min), and MAXX (35% H2O2, 3 x 15 min) groups. At 2 and 30 days, the rats were killed (n = 10). The jaws were processed for histology analysis and PCNA and Caspase-3-cleaved immunohistochemistry, and data were submitted to the Mann-Whitney or ANOVA test (P < 0.05). Results:. At 2 days, the MARX group showed necrosis and the BLUE group revealed moderate inflammation on the occlusal third of the crown (P < 0.05). At 30 days, tertiary dentin had formed and there was an absence of inflammation. The level of cell proliferation was higher in the middle third of the BLUE group (P < 0.05), and cervical of MAXX at 2 days (P < 0.05), decreasing at 30 days. The apoptosis was present at 2 days, particularly in the cervical third of the crown in the bleached groups (P < 0.05), with a decrease only at 30 days in the BLUE group (P < 0.05). Conclusions: The concentration of H2O2 influences effects on the pulp tissue, where a higher concentration of H2O2 can cause necrosis in the pulp and a prolonged effect within the apoptotic process; lower concentrations of H2O2 provide moderate inflammation, cell proliferation and apoptosis with a reduction of these processes over time. (AU)

FAPESP's process: 13/25429-0 - Presence of caspase, PCNA and CD5 in the pulp of rats subjected bleaching with different concentrations hydrogen peroxide: study histological and immunohistochemical
Grantee:Francine Benetti
Support Opportunities: Scholarships in Brazil - Master