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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phytochromobilin deficiency impairs sugar metabolism through the regulation of cytokinin and auxin signaling in tomato fruits

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Author(s):
Bianchetti, Ricardo Ernesto ; Cruz, Aline Bertinatto ; Oliveira, Bruna Soares ; Demarco, Diego ; Purgatto, Eduardo ; Pereira Peres, Lazaro Eustquio ; Rossi, Magdalena ; Freschi, Luciano
Total Authors: 8
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 7, AUG 10 2017.
Web of Science Citations: 9
Abstract

Phytochomes and plant hormones have been emerging as important regulators of fleshy fruit biology and quality traits; however, the relevance of phytochrome-hormonal signaling crosstalk in controlling fruit development and metabolism remains elusive. Here, we show that the deficiency in phytochrome chromophore phytochromobilin (P phi B) biosynthesis inhibits sugar accumulation in tomato (Solanum lycopersicum) fruits by transcriptionally downregulating sink-and starch biosynthesis-related enzymes, such as cell-wall invertases, sucrose transporters and ADP-glucose pyrophosphorylases. PFB deficiency was also shown to repress fruit chloroplast biogenesis, which implicates more limited production of photoassimilates via fruit photosynthesis. Genetic and physiological data revealed the involvement of auxins and cytokinins in mediating the negative impact of PFB deficiency on fruit sink strength and chloroplast formation. PFB deficiency was shown to transcriptionally repress type-A TOMATO RESPONSE REGULATORs and AUXIN RESPONSE FACTORs both in pericarp and columella, suggesting active phytochrome-hormonal signaling crosstalk in these tissues. Data also revealed that PFB deficiency influences fruit ripening by delaying the climacteric rise in ethylene production and signaling. Altogether, the data uncover the impact of phytochromobilin deficiency in fine-tuning sugar metabolism, chloroplast formation and the timing of fruit ripening and also reveal a link between auxins, cytokinins and phytochromes in regulating sugar import and accumulation in fruits. (AU)

FAPESP's process: 13/18056-2 - Interaction between light, hormonal and nitric oxide signaling during plastidial biogenesis and differentiation and nutraceutical compounds accumulation in tomato fruits
Grantee:Luciano Freschi
Support Opportunities: Regular Research Grants
FAPESP's process: 16/01128-9 - Light and hormonal regulation of nutritional quality in Solanum lycopersicum
Grantee:Maria Magdalena Rossi
Support Opportunities: Research Projects - Thematic Grants