Participation of extracellular signal-regulated kinases 1/2 in osteoblast and adipocyte differentiation of mesenchymal stem cells grown on titanium surfaces

Author(s)

Silva, Heitor F. ; Abuna, Rodrigo P. F. ; Lopes, Helena B. ; Francischini, Marcelo S. ; de Oliveira, Paulo T. ; Rosa, Adalberto L. ; Beloti, Marcio M.

• Document type: Journal article
• Source: European Journal of Oral Sciences; v. 125, n. 5, p. 355-360, OCT 2017.
• Web of Science Citations: 4

Abstract

Osteoblasts and adipocytes coexist in the implantation site and affect the process of titanium (Ti) osseointegration. As extracellular signal-regulated kinases 1/2 (ERK1/2) are involved in osteogenesis and adipogenesis, the aim of our study was to investigate if the effects of Ti surface topography on osteoblast and adipocyte differentiation are modulated by ERK1/2. The experiments were conducted based on the effect of the ERK1/2 inhibitor, PD98059, on mesenchymal stem cells (MSCs) grown under osteogenic and adipogenic conditions on Ti with nanotopography (Ti-Nano) or on machined Ti (Ti-Machined). The results showed that, in general, ERK1/2 inhibition favored osteoblast and adipocyte differentiation of MSCs grown on Ti-Machined. In MSCs grown on Ti-Nano, ERK1/2 inhibition upregulated the expression of alkaline phosphatase and osteocalcin and reduced extracellular matrix mineralization. In terms of adipocyte differentiation, ERK1/2 inhibition elicited similar MSC responses to Ti-Nano and Ti-Machined, upregulating gene expression of adipocyte markers without affecting lipid accumulation. Our results indicate that, under osteogenic and adipogenic conditions, the responses of MSCs to Ti surface topography in terms of osteogenesis and adipogenesis are dependent on ERK1/2. Thus, a precise modulation of ERK1/2 expression and activity induced by surface topography could be a good strategy to drive the process of implant osseointegration. (AU)

• FAPESP's process: 16/00182-0 - Participation of focal adhesion kinases in the osteoblast differentiation of mesenchymal stem cells induced by titanium surfaces with micro and nanotopography
• Grantee: Marcelo Soares Francischini
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/01291-6 - Role of the endogenous bone morphogenetic proteins 2 and 4 on the osteoblast phenotype expression in cells grown on titanium with nanotopography
• Grantee: Márcio Mateus Beloti
• Support Opportunities: Regular Research Grants