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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

P2X3 and P2X2/3 Receptors Play a Crucial Role in Articular Hyperalgesia Development Through Inflammatory Mechanisms in the Knee Joint Experimental Synovitis

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Author(s):
Teixeira, Juliana Maia ; Bobinski, Franciane ; Parada, Carlos Amilcar ; Sluka, Kathleen A. ; Tambeli, Claudia Herrera
Total Authors: 5
Document type: Journal article
Source: Molecular Neurobiology; v. 54, n. 8, p. 6174-6186, OCT 2017.
Web of Science Citations: 12
Abstract

Osteoarthritis (OA) is a degenerative and progressive disease characterized by cartilage breakdown and by synovial membrane inflammation, which results in disability, joint swelling, and pain. The purinergic P2X3 and P2X2/3 receptors contribute to development of inflammatory hyperalgesia, participate in arthritis processes in the knee joint, and are expressed in chondrocytes and nociceptive afferent fibers innervating the knee joint. In this study, we hypothesized that P2X3 and P2X2/3 receptors activation by endogenous ATP (adenosine 5'-triphosphate) induces articular hyperalgesia in the knee joint of male and female rats through an indirect sensitization of primary afferent nociceptors dependent on the previous release of pro-inflammatory cytokines and/or on neutrophil migration. We found that the blockade of articular P2X3 and P2X2/3 receptors significantly attenuated carrageenan-induced hyperalgesia in the knee joint of male and estrus female rats in a similar manner. The carrageenan-induced knee joint inflammation increased the expression of P2X3 receptors in chondrocytes of articular cartilage. Further, the blockade of articular P2X3 and P2X2/3 receptors significantly reduced the increased concentration of TNF-alpha, IL-6, and CINC-1 and the neutrophil migration induced by carrageenan. These findings indicate that P2X3 and P2X2/3 receptors activation by endogenous ATP is essential to hyperalgesia development in the knee joint through an indirect sensitization of primary afferent nociceptors dependent on the previous release of pro-inflammatory cytokines and/or on neutrophil migration. (AU)

FAPESP's process: 10/05381-4 - Involvement of P2X3 and P2X7 purinergic receptors in carrageenan-induced arthritis in rat's knee joint
Grantee:Claudia Herrera Tambeli
Support Opportunities: Regular Research Grants
FAPESP's process: 09/16854-3 - Involvement of P2X3 and P2X7 purinergic receptors in carrageenan-induced arthritis in knee joint of rats
Grantee:Juliana Maia Teixeira
Support Opportunities: Scholarships in Brazil - Doctorate