Development of a new model for the study of muscle hyperalgesia and the involvement of P2X3 and P2X2/3 receptors in muscle hyperalgesia

• Grant number: 11/11064-4
• Support Opportunities: Research Grants - Young Investigators Grants
• Duration: April 01, 2012 - March 31, 2014
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Maria Cláudia Gonçalves de Oliveira
• Grantee: Maria Cláudia Gonçalves de Oliveira
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated researchers: Carlos Amilcar Parada ; Suzy Krimon
• Associated scholarship(s): 13/23448-7 - Involvement of the central nervous system receptors in muscle hyperalgesia: role of P2X3, AMPA and NMDA receptors, BP.IC; 13/22792-6 - Role of P2X7 purinergic receptor in muscle pain, BP.IC; 13/22308-7 - Evaluation of muscle injury in a new model of muscle hyperalgesia induced by sustained isometric contraction, BP.IC; 12/10402-6 - Involvement of inflammatory mediators and of P2X3 receptors on muscle hyperalgesia induced by sustained isometric contraction in rats, BP.MS; 11/13884-9 - Mechanisms underlying activation of P2X3 and P2X2/3 receptors induce hyperalgesia in the gastrocnemius muscle of rats, BP.MS

Abstract

Among the kinds of pain that affect people throughout their lives, muscle pain, induced by sustained isometric contraction is one of the most prevalent kinds of pain and has an important socio-economic impact. However, despite their clinical relevance, the molecular mechanisms involved in the development of muscle pain induced by sustained isometric contraction are unknown. This is mainly due to the absence of a more realistic experimental model that has a good degree of prediction of pharmacological control of pain. Studies in humans and animals have provided evidence that endogenous ATP, via activation of P2X3 and P2X2/3 receptors, may induce muscle pain. Therefore, the aim of this study is to develop a new model of muscle hyperalgesia induced by sustained isometric contraction and to study the molecular mechanisms involved in this kind of muscle hyperalgesia, particularly the involvement of P2X3 and P2X2/3.The model is based on the development of a sustained isometric contraction in the gastrocnemius muscle of rats that results in muscle hyperalgesia. The sustained isometric contraction that induces hyperalgesia will be performed by electrical stimulation directly to the belly of the gastrocnemius muscle of rats. Once the model is established, the physiopathologic mechanism of hyperalgesia and the involvement of P2X3 and P2X2/3 receptors will be assessed by techniques of behavioral pharmacology, biochemical analysis and muscle histology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AQUINO, BRUNA DE MELO; DA SILVA DOS SANTOS, DIOGO FRANCISCO; JORGE, CAROLINA OCANHA; SALGADO MARQUES, ALINE CAROLINA; TEIXEIRA, JULIANA MAIA; PARADA, CARLOS AMILCAR; GONCALVES OLIVEIRA-FUSARO, MARIA CLAUDIA. P2X3 receptors contribute to muscle pain induced by static contraction by a mechanism dependent on neutrophil migration. PURINERGIC SIGNALLING, v. 15, n. 2, p. 167-175, JUN 2019. (12/10402-6, 13/23448-7, 11/11064-4)

SANTOS, DIOGO F. S.; MELO-AQUINO, BRUNA; JORGE, CAROLINA O.; CLEMENTE-NAPIMOGA, JULIANA T.; TAYLOR, BRADLEY K.; OLIVEIRA-FUSARO, MARIA C. G.. Prostaglandin 15d-PGJ(2) targets PPAR gamma and opioid receptors to prevent muscle hyperalgesia in rats. Neuroreport, v. 32, n. 3, p. 238-243, FEB 3 2021. (11/11064-4)

CAROLINA OCANHA JORGE; BRUNA DE MELO-AQUINO; DIOGO FRANCISCO DA SILVA DOS SANTOS; MARIA CLÁUDIA GONÇALVES DE OLIVEIRA. Muscle pain induced by static contraction is modulated by transient receptor potential vanilloid 1 and ankyrin 1 receptors. Brazilian Journal of Pharmaceutical Sciences, v. 58, 2022-11-07. (11/11064-4)