Mechanisms underlying activation of P2X3 and P2X2/3 receptors induce hyperalgesia in the gastrocnemius muscle of rats

• Grant number: 11/13884-9
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2012
• Effective date (End): July 31, 2013
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Maria Cláudia Gonçalves de Oliveira
• Grantee: Jalile Garcia Schiavuzzo
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 11/11064-4 - Development of a new model for the study of muscle hyperalgesia and the involvement of P2X3 and P2X2/3 receptors in muscle hyperalgesia, AP.JP

Abstract

Among the kinds of pain that affect people throughout their lives, muscle pain is the most prevalent, however, the mechanisms underlying endogenous ATP via activation of P2X3 and P2X2/3 receptors contributes to development of muscle pain is poorly understood. There is evidence of the involvement of endogenous ATP via activation of P2X3 and P2X2/3 receptors in muscle pain. Recently, we have demonstrated that activation of P2X3 and P2X2/3 receptors by endogenous ATP is essential to development of inflammatory hyperalgesia induced by carrageenan in the subcutaneous tissue of rats hind paw. In other study of our group, we have demonstrated that the mechanical hyperalgesia induced by the non-selective P2X3 receptor agonist, a,b-meATP, in the subcutaneous tissue of rats hind paw occurs by an indirect action on primary afferent nociceptors mediated by prostaglandins, bradykinin, simpathomimetics amines, pro-inflammatory cytokines and neutrophil migration. Although we have demonstrated the mechanism underlying endogenous ATP via activation of P2X3 and P2X2/3 receptors contributes to inflammatory pain in the subcutaneous tissue of rats hind paw, studies suggest that the development of painful processes is mediated by tissue-dependent mechanisms. Therefore, the aim of this study is to verify whether the hyperalgesia induced by activation of P2X3 and P2X2/3 receptors in the gastrocnemius muscle of rats is mediated by prostaglandins, bradykinin and simpathomimetics amines. To this end, we will test the ability of the cyclooxygenase inhibitor, the bradykinin B1 receptor antagonist and beta1 and beta2 adrenergic receptors antagonist to reduce hyperalgesia induced by administration of the P2X3 receptor agonist a,b-meATP in the gastrocnemius muscle of rats.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA DOS SANTOS, DIOGO FRANCISCO; AQUINO, BRUNA DE MELO; JORGE, CAROLINA OCANHA; DE AZAMBUJA, GRACIANA; SCHIAVUZZO, JALILE GARCIA; KRIMON, SUZY; NEVES, JULIANA DOS SANTOS; PARADA, CARLOS AMILCAR; GOCALVES OLIVEIRA-FUSARO, MARIA CLAUDIA. MUSCLE PAIN INDUCED BY STATIC CONTRACTION IN RATS IS MODULATED BY PERIPHERAL INFLAMMATORY MECHANISMS. Neuroscience, v. 358, p. 58-69, SEP 1 2017. (12/10402-6, 11/13884-9)

SCHIAVUZZO, J. G.; TEIXEIRA, J. M.; MELO, B.; DA SILVA DOS SANTOS, D. F.; JORGE, C. O.; OLIVEIRA-FUSARO, M. C. G.; PARADA, C. A.. MUSCLE HYPERALGESIA INDUCED BY PERIPHERAL P2X3 RECEPTORS IS MODULATED BY INFLAMMATORY MEDIATORS. Neuroscience, v. 285, p. 24-33, JAN 29 2015. (11/13884-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SCHIAVUZZO, Jalile Garcia. Mechanisms underling the role of P2X3 and P2X2/3 receptors in mechanical hyperalgesia in gastrocnemius muscle of rats. 2013. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas Limeira, SP.