Interaction between neurons and satellite cells in dorsal root ganglia: ATP signaling to the development of inflammatory and neuropathic hyperalgesia.

• Grant number: 17/23485-0
• Support Opportunities: Regular Research Grants
• Duration: March 01, 2018 - February 29, 2020
• Field of knowledge: Biological Sciences - Physiology
• Principal Investigator: Carlos Amilcar Parada
• Grantee: Carlos Amilcar Parada
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The dorsal root ganglion is an important site of pain modulation. Studies from our and others laboratories have been suggested that the interaction between neurons and glial satellite cells is important in the development of inflammatory hyperalgesia as well as neuropathic hyperalgesia. Many molecules involved in this interaction emerge as a promising pharmacological target to differently control inflammatory pain and neuropathic pain, among them the ATP. This molecule initially known as an intracellular energy source also plays an important role in intracellular and intercellular signaling under physiological and pathological conditions. In the dorsal root ganglion, the ATP can be released by the body of the sensory neurons through lysosomal exocytosis or through the membrane channels called panexins (panx). Currently ATP is considered one of the most important molecules in the neuron-glia interaction in the sensory ganglia. In vivo and in vitro assays performed by our group suggest that the ATP acting in P2X7 receptor is important to development of inflammatory hyperalgesia. However, to the development of neuropathic hyperalgesia, the activation of P2X4 receptor seems to be more important. Therefore, this project is divided in two sub-projects. The first one intends to study the participation of C fibers, P2X4 and P2X7 receptors in the development of inflammatory hyperalgesia induced by carrageenan and PGE2, and neuropathic hyperalgesia induced by paclitaxel. The second one aims to study the signaling of ATP released in the dorsal root ganglion in the development of inflammatory hyperalgesia induced by carrageenan and also in paclitaxel-induced neuropathy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MAGALHAES, SILVIANE F.; MANZO, LUIS P.; DE FARIA, FELIPE M.; DE OLIVEIRA-FUSARO, MARIA C.; NISHIJIMA, CATARINE M.; VIEIRA, WILLIANS F.; BONET, IVAN J. M.; DOS SANTOS, GILSON G.; TAMBELI, CLAUDIA H.; PARADA, CARLOS A.. Inflammatory pain in peripheral tissue depends on the activation of the TNF-alpha type 1 receptor in the primary afferent neuron. European Journal of Neuroscience, v. 53, n. 2, p. 376-389, JAN 2021. (14/25153-7, 17/23485-0)