Involvement of inflammatory mediators and of P2X3 receptors on muscle hyperalgesia induced by sustained isometric contraction in rats

• Grant number: 12/10402-6
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): September 01, 2012
• Effective date (End): February 28, 2014
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Maria Cláudia Gonçalves de Oliveira
• Grantee: Bruna de Melo Aquino
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 11/11064-4 - Development of a new model for the study of muscle hyperalgesia and the involvement of P2X3 and P2X2/3 receptors in muscle hyperalgesia, AP.JP

Abstract

Among the kinds of pain that affect people throughout their lives, muscle pain, specially the one induced by sustained isometric contraction, is one of the most prevalent kinds of pain and has an important socio-economic impact. However, despite their clinical relevance, the inflammatory molecular mechanisms involved in the development of muscle pain induced by sustained isometric contraction are unknown. This is mainly due to the absence of a more realistic experimental model that has a good degree of prediction of pharmacological control of pain. Studies in humans and animals have provided evidence that endogenous ATP, via activation of P2X3 and P2X2/3 receptors, may induce muscle pain. Therefore, the aim of this study is to study the inflammatory molecular mechanisms involved in muscle hyperalgesia induced by sustained isometric contraction, particularly the involvement of P2X3 and P2X2/3. The sustained isometric contraction will be performed by electrical stimulation directly to the belly of the gastrocnemius muscle of rats and the inflammatory molecular mechanisms, specially the involvement of P2X3 receptors, will be analyzed by behavior pharmacology and biochemical analysis of cytokines and neutrophil migration.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AQUINO, BRUNA DE MELO; DA SILVA DOS SANTOS, DIOGO FRANCISCO; JORGE, CAROLINA OCANHA; SALGADO MARQUES, ALINE CAROLINA; TEIXEIRA, JULIANA MAIA; PARADA, CARLOS AMILCAR; GONCALVES OLIVEIRA-FUSARO, MARIA CLAUDIA. P2X3 receptors contribute to muscle pain induced by static contraction by a mechanism dependent on neutrophil migration. PURINERGIC SIGNALLING, v. 15, n. 2, p. 167-175, JUN 2019. (12/10402-6, 13/23448-7, 11/11064-4)

DA SILVA DOS SANTOS, DIOGO FRANCISCO; AQUINO, BRUNA DE MELO; JORGE, CAROLINA OCANHA; DE AZAMBUJA, GRACIANA; SCHIAVUZZO, JALILE GARCIA; KRIMON, SUZY; NEVES, JULIANA DOS SANTOS; PARADA, CARLOS AMILCAR; GOCALVES OLIVEIRA-FUSARO, MARIA CLAUDIA. MUSCLE PAIN INDUCED BY STATIC CONTRACTION IN RATS IS MODULATED BY PERIPHERAL INFLAMMATORY MECHANISMS. Neuroscience, v. 358, p. 58-69, SEP 1 2017. (12/10402-6, 11/13884-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

AQUINO, Bruna de Melo. The inflammatory mechanisms involved in mechanical muscle hyperalgesia induced by susteined isometric contraction in rats. 2014. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas Limeira, SP.