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| Author(s): |
Bruna de Melo
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Limeira, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas |
| Defense date: | 2014-02-28 |
| Examining board members: |
Maria Cláudia Gonçalves de Oliveira;
Juliana Trindade Clemente Napimoga;
Dionéia Araldi
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| Advisor: | Maria Cláudia Gonçalves de Oliveira |
| Abstract | |
Studies show that muscle pain induced by sustained isometric contraction (CIS) have an important socioeconomic impact, however, despite their clinical relevance, the inflammatory mechanisms involved in the development of this type of pain are still poorly understood. The aim of this study was to analyze the inflammatory mechanisms involved in the development of this type of muscle hyperalgesia. For this model of muscle hyperalgesia induced by sustained isometric contraction, recently developed by our research group, which consists of the induction of isometric muscle contraction in the gastrocnemius muscle of male Wistar rats were used, weighing between 200 and 250g, receiving an electric current through the equipment brand Grass , model SX88R , single phase , repeated pulse frequency of 50 Hz , pulse duration of 19ms via needle-like electrodes for a period of 1 hour. To trace the inflammatory profile of this model, the following drugs were administered intramuscularly 5 minutes before the isometric contraction: DALB K (3; 30'mu'g) and Bradizyde (1.5, 15 mg) (antagonists of bradykinin B1 and B2 receptors, respectively), Atenolol (0.6 ; 6'mu'g) and ICI 118551 (0.15, 1.5 mg) ('beta1 and 'beta'2 adrenergic receptor antagonists, respectively), indomethacin (10 ; 100'mu'g ) ( inhibitory action of cyclooxygenase ) and A317491 ( 0.6 , 6, 60 mg) (selective antagonist of P2X3 and P2X2 / 3 receptors) , and administered intraperitoneally 20 minutes before the fucoidin ( 25mg/Kg ) ( inhibitory action of selectins) . The results showed that all of these antagonists and inhibitors significantly reduced muscular hyperalgesia induced by sustained isometric contraction, confirming the involvement of bradykinin, sympathomimetic amines, prostaglandins, neutrophils and endogenous ATP via P2X3 and P2X2/3 receptors in muscle hyperalgesia induced by isometric contraction sustained. These results delineate first the inflammatory profile of muscle hyperalgesia induced by sustained isometric contraction and suggest important targets for study and treatment of muscle pain, and open new perspectives for the study of other important mechanisms in the development of the same (AU) | |
| FAPESP's process: | 12/10402-6 - Involvement of inflammatory mediators and of P2X3 receptors on muscle hyperalgesia induced by sustained isometric contraction in rats |
| Grantee: | Bruna de Melo Aquino |
| Support Opportunities: | Scholarships in Brazil - Master |