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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

UV/Vis spectroscopy combined with chemometrics for monitoring solid-state fermentation with Rhizopus microsporus var. oligosporus

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Author(s):
Ito, Shuri ; Barchi, Augusto Cesar ; Escaramboni, Bruna ; de Oliva Neto, Pedro ; Herculano, Rondinelli Donizetti ; Borges, Felipe Azevedo ; Romeiro Miranda, Matheus Carlos ; Fernandez Nunez, Eutimio Gustavo
Total Authors: 8
Document type: Journal article
Source: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY; v. 92, n. 10, p. 2563-2572, OCT 2017.
Web of Science Citations: 2
Abstract

BACKGROUNDDifficulties in bioprocess monitoring are a drawback of solid-state fermentation (SSF). Specifically, monitoring of enzyme activities in SSF is not an easy task. This work aimed to calibrate partial least squares (PLS) and artificial neural network (ANN) models for inferring protease and amylase activities, as well as protein concentration, from UV-Vis spectra of aqueous extracts of samples removed during SSF using Rhizopus microsporus var. oligosporus. RESULTSSSFs were performed using single agro-industrial wastes (wheat bran, type II wheat flour, sugarcane bagasse and soybean meal) and ternary mixtures of them. Enzyme activities and protein concentrations in the aqueous extracts were quantified biochemically. The corresponding UV-Vis spectra of diluted extracts were also collected. The prediction quality of the ANN was higher than that of the PLS model. The relative errors considering the range for amylolytic and proteolytic enzymes were 4% (3-442 U g(-1)) and 6% (0-256 U g(-1)), respectively, for the best ANN architectures (8 and 6 neurons in hidden layer, respectively). CONCLUSIONThese results, in combination with correlation coefficients (R>0.94), suggest that this approach is suitable for developing a chemosensor for monitoring SSFs, reducing the analytical work for quantification of enzyme activities. No satisfactory results were obtained for protein concentration. (c) 2017 Society of Chemical Industry (AU)

FAPESP's process: 14/06447-0 - Establishing of chemometric technique for enzyme activities quantification based on FT-IR spectroscopy and artificial neural networks
Grantee:Augusto Cesar Barchi
Support Opportunities: Scholarships in Brazil - Scientific Initiation