Preferential Mitochondrial Localization of a Goniothalamin Fluorescent Derivative

Author(s)

Raitz, Ismael ; de Souza Filho, Roberto Y. ; de Andrade, Lorena P. ; Correa, Jose R. ; Neto, Brenno A. D. ; Pilli, Ronaldo A.

• Document type: Journal article
• Source: ACS OMEGA; v. 2, n. 7, p. 3774-3784, JUL 2017.
• Web of Science Citations: 3

Abstract

A fluorescent 2,1,3-benzothiadiazole- containing goniothalamin derivative, BTD-GTN (1), has been synthesized and successfully tested in bioimaging experiments in live cells. The fluorescent compound proved to be capable of transposing the cell membranes, indicating its subcellular localization. The use of the benzothiadiazole core as the fluorophore revealed the favored localization of the GTN analogue 1 in the cytoplasm of live cells, preferentially in the mitochondria, in line with previous results that indicated the loss of mitochondrial transmembrane potential upon treatment with GTN. The results described herein highlight the potential of the BTD-GTN hybrid structures for future studies regarding the cellular mechanism of action of this family of compounds. (AU)

• FAPESP's process: 09/51602-5 - Chemical biology: new natural and synthetic molecular targets against cancer, structural studies, biological evaluation and mode of action
• Grantee: Ronaldo Aloise Pilli
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/12541-4 - A biomimetic approach to the total synthesis of Stemona alkaloids
• Grantee: Ronaldo Aloise Pilli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center
• Grantee: Licio Augusto Velloso
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC