Ruthenium Complex Improves the Endothelial Function in Aortic Rings From Hypertensive Rats

Author(s)

Vatanabe, Izabela Pereira ; dos Santos Rodrigues, Carla Nascimento ; Buzinari, Tereza Cristina ; de Moraes, Thiago Francisco ; da Silva, Roberto Santana ; Rodrigues, Gerson Jhonatan

• Document type: Journal article
• Source: Arquivos Brasileiros de Cardiologia; v. 109, n. 2, p. 124-131, AUG 2017.
• Web of Science Citations: 1

Abstract

Background: The endothelium is a monolayer of cells that extends on the vascular inner surface, responsible for the modulation of vascular tone. By means of the release of nitric oxide (NO), the endothelium has an important protective function against cardiovascular diseases. Objective: Verify if cis-{[}Ru(bpy)(2)(NO2)(NO)](PF6)(2)(BPY) improves endothelial function and the sensibility of conductance (aorta) and resistance (coronary) to vascular relaxation induced by BPY. Methods: Normotensive (2K) and hypertensive (2K-1C) Wistar rats were used. For vascular reactivity study, thoracic aortas were isolated, rings with intact endothelium were incubated with: BPY(0.01 to10 mu M) and concentration effect curves to acetylcholine were performed. In addition, cumulative concentration curves were performed to BPY (1.0 nM to 0.1 mu M) in aortic and coronary rings, with intact and denuded endothelium. Results: In aorta from 2K-1C animals, the treatment with BPY 0.1 mu M increased the potency of acetylcholine-induced relaxation and it was able to revert the endothelial dysfunction. The presence of the endothelium did not modify the effect of BPY in inducing the relaxation in aortas from 2K and 2K-1C rats. In coronary, the endothelium potentiated the vasodilator effect of BPY in vessels from 2K and 2K-1C rats. Conclusion: Our results suggest that 0.1 mu M of BPY is able to normalize the relaxation endothelium dependent in hypertensive rats, and the compound BPY induces relaxation in aortic from normotensive and hypertensive rats with the same potency. The endothelium potentiate the relaxation effect induced by BPY in coronary from normotensive and hypertensive rats, with lower effect on coronary from hypertensive rats. (AU)

• FAPESP's process: 14/02231-2 - Use of ruthenium complex cis-[Ru (bpy) 2 (NO2) (NO)] (PF6) 2) to reverse and / or prevent endothelial dysfunction in arterial hypertension
• Grantee: Izabela Pereira Vatanabe
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/24477-8 - Utilization of ruthenium complex as pharmacological strategy to revert and/or prevent the endothelial dysfunction
• Grantee: Gerson Jhonatan Rodrigues
• Support Opportunities: Research Grants - Young Investigators Grants