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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Reduced glucose-induced insulin secretion in low-protein-fed rats is associated with altered pancreatic islets redox status

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Author(s):
Cappelli, Ana Paula G. [1, 2, 3] ; Zoppi, Claudio C. [1] ; Silveira, Leonardo R. [1] ; Batista, Thiago M. [1] ; Paula, Flavia M. [1] ; da Silva, Priscilla M. R. ; Rafacho, Alex [1, 4] ; Barbosa-Sampaio, Helena C. [1] ; Boschero, Antonio C. [1] ; Carneiro, Everardo M. [1]
Total Authors: 10
Affiliation:
[1] Univ Campinas UNICAMP, Dept Struct & Funct Biol Cellular Biol & Physiol, Inst Biol, Campinas, SP - Brazil
[2] Fed Univ Maranhao UFMA, Lab Expt Physiol, Dept Physiol Sci, Sao Luis, Maranhao - Brazil
[3] Univ Sao Paulo, Dept Physiol & Biophysiol, Inst Biomed Sci, Sao Paulo - Brazil
[4] Fed Univ Santa Catarina UFSC, Dept Physiol Sci, Ctr Biol Sci, Florianopolis, SC - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Cellular Physiology; v. 233, n. 1, p. 486-496, JAN 2018.
Web of Science Citations: 3
Abstract

In the present study, we investigated the relationship between early life protein malnutrition-induced redox imbalance, and reduced glucose-stimulated insulin secretion. After weaning, male Wistar rats were submitted to a normal-protein-diet (17%-protein, NP) or to a low-protein-diet (6%-protein, LP) for 60 days. Pancreatic islets were isolated and hydrogen peroxide (H2O2), oxidized (GSSG) and reduced (GSH) glutathione content, CuZn-superoxide dismutase (SOD1), glutathione peroxidase (GPx1) and catalase (CAT) gene expression, as well as enzymatic antioxidant activities were quantified. Islets that were pre-incubated with H2O2 and/or N-acetylcysteine, were subsequently incubated with glucose for insulin secretion measurement. Protein malnutrition increased CAT mRNA content by 100%. LP group SOD1 and CAT activities were 50% increased and reduced, respectively. H2O2 production was more than 50% increased whereas GSH/GSSG ratio was near 60% lower in LP group. Insulin secretion was, in most conditions, approximately 50% lower in LP rat islets. When islets were pre-incubated with H2O2 (100M), and incubated with glucose (33mM), LP rats showed significant decrease of insulin secretion. This effect was attenuated when LP islets were exposed to N-acetylcysteine. (AU)

FAPESP's process: 07/50365-4 - Study of the destruction mechanisms of beta pancreatic cells during the onset of Diabetes Mellitus (DM2): search for inhibition strategies of this process as well as for the recovery of insular mass in different animal models
Grantee:Antonio Carlos Boschiero
Support Opportunities: Research Projects - Thematic Grants