Idiopathic atrophoderma of Pasini and Pierini: A case study of collagen and elastin texture by multiphoton microscopy

Author(s)

Vieira-Damiani, Gislaine ^[1] ; Lage, Denise ^[2] ; Christofoletti Daldon, Patricia Erica ^[2] ; Tiburcio Alves, Caroline Romanelli ^[2] ; Cintra, Maria Leticia ^[1] ; Metze, Konradin ^[1] ; Adur, Javier ^[3] ; Pelegati, Vitor B. ^[3] ; Carvalho, Hernandes F. ^{[3, 4, 5]} ; Cesar, Carlos Lenz ^{[6, 3, 4]}

Affiliation

^[1] Inst Phys Gleb Wataghin, Dept Pathol, Med Sch, Campinas, SP - Brazil

^[2] Pontifical Catholic Univ Campinas, Celso Pierro Hosp, Dept Dermatol, Campinas, SP - Brazil

^[3] Inst Phys Gleb Wataghin, Biophoton Grp, Dept Quantum Elect, Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Natl Inst Sci & Technol Photon Appl Cell Biol, Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Struct & Funct Biol, Inst Biol, Campinas, SP - Brazil

^[6] Univ Fed Ceara, Dept Phys, Fortaleza, Ceara - Brazil

• Document type: Journal article
• Source: JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY; v. 77, n. 5, p. 930-937, NOV 2017.
• Web of Science Citations: 6

Abstract

Background: The diagnosis of idiopathic atrophoderma of Pasini and Pierini (IAPP) relies on typical clinical features, particularly distinctive pigmented ovular/round depressed plaques. Histologic examination often reveals no obvious changes, but patterns of collagen distribution, using multiphoton imaging and second harmonic generation can help track hidden details of tissue organization contributing to atrophy. Objective: To identify histologic features that distinguish IAPP from unaffected skin. Methods: Eleven patients were included for conventional analyses. Masson trichromee and Unna-Tanzer orcein-stained sections were evaluated using automated morphometry. Hematoxylin-eosinestained sections were analyzed by multiphoton imaging using 2-photon excited fluorescence and second harmonic generation. Results: No abnormalities were found under light microscopy or by automated quantification. Multiphoton imaging revealed no difference in optical density of either collagen or elastic fibers in lesioned and unaffected skin; however, horizontal collagen fiber organization in lesion specimens increased toward the lower dermis, whereas elastic fibers featured greater disorganization within the upper dermis. Limitations: The low number of patients evaluated. Conclusion: The atrophic appearance of IAPP lesions reflects changes in organization, but not in collagen and elastic tissue content. Minute organizational differences that are imperceptible to the experienced pathologist and undetectable by automated analyses were revealed by multiphoton analyses, particularly second harmonic generation, in association with texture analyses. (AU)

• FAPESP's process: 11/51959-0 - Biology of neoplastic diseases of bone marrow
• Grantee: Sara Teresinha Olalla Saad
• Support Opportunities: Research Projects - Thematic Grants