Vitamin E treatment decreases muscle injury in mdx mice

Author(s)

Mancio, Rafael Dias ^[1] ; Hermes, Tulio de Almeida ^[1] ; Macedo, Aline Barbosa ^[1] ; Mizobuti, Daniela Sayuri ^[1] ; Valduga, Amanda Harduim ^[1] ; Rupcic, Ian Feller ^[1] ; Minatel, Elaine ^[1]

Affiliation

^[1] State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil

• Document type: Journal article
• Source: NUTRITION; v. 43-44, p. 39-46, NOV-DEC 2017.
• Web of Science Citations: 3

Abstract

Objective: Oxidative stress, in addition to the absence of the dystrophin protein, has been considered an important regulator of Duchenne muscular dystrophy (DMD). Vitamin E presents an important role as a potent antioxidant and in preserving the integrity of the cell membrane. In this study, we evaluated the effects of vitamin E therapy on some physiological pathways that can contribute to muscle injury in the diaphragm muscle of mdx mice (the experimental model of DMD) such as CK levels, inflammatory response, oxidative stress, and the enzymatic antioxidant system. Methods: Mdx mice (14 d old) received 40 mg vitamin E/kg daily by oral gavage for 14 d, followed by the removal of the diaphragm muscle. Control mdx mice and C57BL/10 mice received saline only for the same period and were used as controls. Results: Vitamin E reduced the muscle fiber damage, oxidative stress, and inflammation process in the diaphragm muscle of mdx mice. Conclusions: Vitamin E improves skeletal muscle injury in mdx mice, promoting membrane repair and exhibiting antioxidant and antiinflammatory effects. These vitamin E effects suggest that this antioxidant therapy may be a relevant approach for dystrophinopathies. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 14/01970-6 - Effect of Diacerein and vitamin E on dystrophic muscle fibers of mdx mice
• Grantee: Rafael Dias Mâncio
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/17732-4 - In vivo and in vitro effect of the antiinflammatory and antioxidant on dystrophic muscle fibers of Mdx mice
• Grantee: Elaine Minatel
• Support Opportunities: Regular Research Grants