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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of growth hormone on cardiac remodeling and soleus muscle in rats with aortic stenosis-induced heart failure

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Author(s):
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Lima, Aline R. R. [1] ; Pagan, Luana U. [1] ; Damatto, Ricardo L. [1] ; Cezar, Marcelo D. M. [1] ; Bonomo, Camila [1] ; Gomes, Mariana J. [1] ; Martinez, Paula F. [2] ; Guizoni, Daniele M. [1] ; Campos, Dijon H. S. [1] ; Damatto, Felipe C. [1] ; Okoshi, Katashi [1] ; Okoshi, Marina P. [1]
Total Authors: 12
Affiliation:
[1] Sao Paulo State Univ, Botucatu Med Sch, Internal Med Dept, UNESP, Botucatu, SP - Brazil
[2] Univ Fed Mato Grosso do Sul, Sch Phys Therapy, Campo Grande - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ONCOTARGET; v. 8, n. 47, p. 83009-83021, OCT 10 2017.
Web of Science Citations: 2
Abstract

Background: Skeletal muscle wasting is often observed in heart failure (HF). The growth hormone (GH)/insulin-like growth factor-1 (IGF-1) axis is impaired in HF. In this study, we evaluated the effects of GH on soleus muscle and cardiac remodeling in rats with aortic stenosis (AS)-induced HF. Methods: AS was created by placing a stainless-steel clip on the ascending aorta. After clinically detecting HF, GH (2 mg/kg/day) was subcutaneously injected for 14 days (AS-GH group). Results were compared with those from Sham and non-treated AS groups. Transthoracic echocardiogram was performed before and after treatment. Protein expression was evaluated by Western blot and satellite cells activation by immunofluorescence. Statistical analyzes: ANOVA and Tukey or Kruskal-Wallis and Student-Newman-Keuls. Results: Before treatment both AS groups presented a similar degree of cardiac injury. GH prevented body weight loss and attenuated systolic dysfunction. Soleus cross-sectional fiber areas were lower in both AS groups than Sham (Sham 3,556 +/- 447; AS 2,882 +/- 422; AS-GH 2,868 +/- 591 mu m(2); p=0.016). GH increased IGF1 serum concentration (Sham 938 +/- 83; AS 866 +/- 116; AS-GH 1167 +/- 166 ng/mL; p< 0.0001) and IGF-1 muscle protein expression and activated PI3K protein. Neural cell adhesion molecule (NCAM) immunofluorescence was increased in both AS groups. Catabolism-related intracellular pathways did not differ between groups. Conclusion: Short-term growth hormone attenuates left ventricular systolic dysfunction in rats with aortic stenosis-induced HF. Despite preserving body weight, increasing serum and muscular IGF-1 levels, and stimulating PI3K muscle expression, GH does not modulate soleus muscle trophism, satellite cells activation or intracellular pathways associated with muscle catabolism. (AU)

FAPESP's process: 09/54102-3 - Acquisition of an ultracentrifuge and an echocardiograph to equip a Centralized Multi-User Laboratory
Grantee:Marina Politi Okoshi
Support type: Multi-user Equipment Program
FAPESP's process: 12/50512-5 - Effects of physical training on throphism and myostatin/follistatin pathway in skeletal and cardiac muscle of spontaneously hypertensive rats with heart failure
Grantee:Marina Politi Okoshi
Support type: Regular Research Grants