Tetracarboxy-phthalocyanines: From excited state dynamics to photodynamic inactivation against Bovine herpesvirus type 1

Author(s):Show less -

Cocca, Leandro H. Z. ^[1] ; Oliveira, Taise M. A. ^[2] ; Gotardo, Fernando ^[1] ; Teles, Amanda V. ^[2] ; Menegatti, Ricardo ^[3] ; Siqueira, Jonathas P. ^[1] ; Mendonca, Cleber R. ^[1] ; Bataus, Luiz A. M. ^[2] ; Ribeiro, Anderson O. ^[4] ; Souza, Thalita F. M. ^[4] ; Souza, Guilherme R. L. ^{[2, 5]} ; Goncalves, Pablo J. ^{[6, 7]} ; De Boni, Leonardo ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Fis Sao Carlos, Sao Carlos, SP - Brazil

^[2] Univ Fed Goias, Inst Ciencias Biol, Goiania, Go - Brazil

^[3] Univ Fed Goias, Inst Farm, Goiania, Go - Brazil

^[4] Fed Univ ABC UFABC, Ctr Nat Sci & Humanities, Santo Andre, SP - Brazil

^[5] Univ Fed Goias, Programa Posgrad Ciencia Anim, Goiania, Go - Brazil

^[6] Univ Fed Goias, Inst Fis, Goiania, Go - Brazil

^[7] Univ Fed Goias, Programa Posgrad Quim, Goiania, Go - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 175, p. 1-8, OCT 2017.
• Web of Science Citations: 4

Abstract

Herein we present the excited state dynamic of zinc and aluminum tetracarboxy-phthalocyanines (ZnPc and AlPc) and its application in the photodynamic inactivation (PDI) of Bovine herpesvirus type 1 (BoHV-1) in vitro. The excited state dynamic provides valuable data to describe the excited state properties of potential optical limiters and/or photosensitizers (PSs), such as: the excited state cross-sections, fluorescence lifetime and triplet state quantum yield. The excited state characterization was performed using three different Z-scan techniques: Single Pulse, White Light Continuum and Pulse Train. Considering the photodynamic inactivation of BoHV-1, an initial viral suspension containing 10(5.75) TCID50/mL was incubated with the PSs for 1 h at 37 degrees C under agitation and protected from light. The samples were placed in microtiter plates and irradiated (180 mW/cm(2)). During irradiation, a sample was taken every 15 min and the viability of the virus was evaluated. The results show that both phthalocyanines were efficient against viruses. However, a higher photodynamic efficiency was observed by ZnPc, which can be attributed to its higher triplet and singlet quantum yields. The results presented here are important for animal health (treatment of BoHV-1) and also open up a field of studies to use AlPc and ZnPc as potential agents against a wide range of microorganisms of veterinary interest. (AU)

• FAPESP's process: 11/12399-0 - Femtosecond pulses applied to nonlinear optics: spectroscopy, pulse shaping and microfabrication
• Grantee: Cleber Renato Mendonça
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 16/20886-1 - Ultrafast nonlinear optical spectroscopy: Transient Absorption and optical Kerr Gate with polarization control
• Grantee: Leonardo De Boni
• Support type: Regular Research Grants