| Full text | |
| Author(s): Show less - |
Rocha, Liliam K. H.
[1]
;
Favaro, Laura I. L.
[1]
;
Rios, Alessandra C.
[1]
;
Silva, Erica C.
[1]
;
Silva, Welida F.
[1]
;
Stigliani, Tatiane P.
[1]
;
Gunger, Mariana
[1]
;
Lima, Renata
[1]
;
Oliveira, Jr., Jose M.
[1]
;
Aranha, Norberto
[1]
;
Tubino, Matthieu
[2]
;
Vila, Marta M. D. C.
[1]
;
Balcao, Victor M.
[1, 3]
Total Authors: 13
|
| Affiliation: | [1] Univ Sorocaba, UNISO, PhageLab Lab Biofilms & Bacteriophages, Intelligent Biosensing & Biom Stabilizat Res Grp, Sorocaba, SP - Brazil
[2] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil
[3] Univ Minho, CEB, Ctr Biol Engn, Braga - Portugal
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Process Biochemistry; v. 61, p. 163-177, OCT 2017. |
| Web of Science Citations: | 8 |
| Abstract | |
In the present research effort, production of crude sericin extracts from Bombyx mori silk cocoons was attempted using two different approaches. Sericin was extracted from cocoons by high-temperature autoclaving followed either by lyophilization or freezing-thawing precipitation, to obtain a crude sericin powder. The physicochemical and biological characteristics of the crude sericin extracts were evaluated in detail, via FTIR, XRD, XRF, XRT, UV-vis scanning, TGA and DSC, protein quantification, antimicrobial activity, free radical scavenging activity, cytotoxic activity, potential for inducing chromosomal aberrations via Allium cepa assays, and genotoxicity via Comet (TM) analyses. The molecular weight distribution of the crude sericin extracts was also investigated, via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the results duly compared to standard sericin. The results gathered clearly suggest that the crude sericin extracts had both obvious radical scavenging effects with the 2.2-diphenyl-1-picryl-hydrazil (DPPH) assay, and antibacterial activity, further suggesting that this protein might be a valuable addition for either food and biopharmaceutical applications. (AU) | |
| FAPESP's process: | 16/16641-3 - Structural and functional stabilization of sericin in a biopolysaccharide hydrogel: bio-origami for skin regeneration |
| Grantee: | Liliam Katsue Harada Rocha |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/12234-4 - TransAppIL - Transdermal delivery of structurally and functionally stabilized protein entities applying ionic liquids. |
| Grantee: | Marta Maria Duarte Carvalho Vila |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/08884-3 - PneumoPhageColor - development of a colorimetric biodetection kit for Pseudomonas aeruginosa based on phage particles |
| Grantee: | Vitor Manuel Cardoso Figueiredo Balcão |
| Support Opportunities: | Regular Research Grants |