Radiofrequency treatment induces fibroblast growth factor 2 expression and subsequently promotes neocollagenesis and neoangiogenesis in the skin tissue

Author(s):Show less -

Meyer, Patricia Froes ^[1] ; de Oliveira, Patricia ^[1] ; Silva, Flavia K. B. A. ^[1] ; da Costa, Ana C. S. ^[1] ; Pereira, Caline R. A. ^[1] ; Casenave, Sebastian ^[2] ; Valentim Silva, Rodrigo Marcel ^[3] ; Araujo-Neto, Luis Gonzaga ^[1] ; Santos-Filho, Sebastiao David ^[4] ; Aizamaque, Eric ^[1] ; Araujo, Hennes Gentil ^[1] ; Bernardo-Filho, Mario ^[4] ; Freire Carvalho, Maria Goretti ^[5] ; Soares, Ciro Dantas ^{[5, 6, 7]}

Affiliation

^[1] Univ Potiguar, Dept Physiotherapy, Natal, RN - Brazil

^[2] Cordoba Univ, Cordoba - Argentina

^[3] Fed Univ, Dept Physiotherapy, Natal, RN - Brazil

^[4] State Univ, Biophys & Biometry Dept, Urca, RJ - Brazil

^[5] Univ Potiguar, Dept Med, Pathol Area, Natal, RN - Brazil

^[6] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Oral Diag, Oral Pathol, Piracicaba, SP - Brazil

^[7] Univ Estadual Campinas, Dent Sch Piracicaba, Pathol Dept, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 32, n. 8, p. 1727-1736, NOV 2017.
• Web of Science Citations: 4

Abstract

Radiofrequency (RF) treatment appears to be involved in production of new collagen fibrils and the improvement of existing collagen structures; however, the molecular bases of the effect of non-invasive RF on the skin tissue have not been fully elucidated. This study reports the effects of RF associated or not with hydrolyzed collagen (HC) in the skin tissue. Wistar rats were randomly divided into four groups, according to the treatment received: control group (G1, n = 5), no treatment; subjects in groupG2 (n = 5) were treated with HC; and capacitive RF was applied to the back of each subject in G3 (n = 5) and RF associated with HC in G4 (n = 5). Biopsies were taken 30 days after treatment and then were histologically processed and studied for inflammatory cell counting, collagen content, and morphometry. In addition, FGF2, CD105, and COX-2 expression was assessed by immunohistochemical staining. The most relevant changes were the increase in cellularity and accumulation of intercellular substance in RF-treated animals (G3 and G4). The greatest dermis thickness rate was observed in G4, followed by G3 and G2 (p < 0.05). RF-treated skins (G3 and G4) exhibited a significant overexpression of FGF2 (p < 0.0001) and increased microvessel density (p < 0.0001) in comparison with G1 and G2. Moreover, the amount of COX-2 was significantly higher (p < 0.0001) in dermis of RF-treated areas compared to G1 and G2, and demonstrated differences in G3 (RF) compared to G4 (RF + HC) (p < 0.0001). Our results suggests that RF treatment associated or not with HC induces FGF2 overexpression, promotes neoangiogenesis and modulates the COX-2 expression, subsequently promotes neocollagenesis, and increased thickness rate of dermis. (AU)

• FAPESP's process: 15/25905-1 - Akt, COX-2, RUNX1 and MMPs expression in metastatic melanomas and Biotechnology prospection of molecules with antineoplastic activity
• Grantee: Ciro Dantas Soares
• Support Opportunities: Scholarships in Brazil - Doctorate