Production of recombinant coagulation factors: Are humans the best host cells?

Author(s)

Swiech, Kamilla ^{[1, 2]} ; Picanco-Castro, Virginia ^[2] ; Covas, Dimas Tadeu ^{[3, 2]}

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Reg Blood Ctr Ribeirao Preto, Ctr Cell Based Therapy, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Sao Paulo - Brazil

• Document type: Review article
• Source: BIOENGINEERED; v. 8, n. 5, p. 462-470, 2017.
• Web of Science Citations: 4

Abstract

The main treatment option for Hemophilia A/B patients involves the administration of recombinant coagulation factors on-demand or in a prophylactic approach. Despite the safety and efficacy of this replacement therapy, the development of antibodies against the coagulation factor infused, which neutralize the procoagulant activity, is a severe complication. The production of recombinant coagulation factors in human cell lines is an efficient approach to avoid such complication. Human cell lines can produce recombinant proteins with post translation modifications more similar to their natural counterpart, reducing potential immunogenic reactions. This review provides a brief overview of the most important characteristics of recombinant FVIII and FIX products available on the market and the improvements that have recently been achieved by the production using human cell lines. (AU)

• FAPESP's process: 12/04629-8 - Establishment of a production platform for recombinant therapeutic proteins in human cells
• Grantee: Kamilla Swiech Antonietto
• Support type: Research Grants - Young Investigators Grants
• FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
• Grantee: Dimas Tadeu Covas
• Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC