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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of nerolidol and limonene on stratum corneum membranes: A probe EPR and fluorescence spectroscopy study

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Author(s):
Mendanha, Sebastiao Antonio [1] ; Marquezin, Cassia Alessandra [1] ; Ito, Amando Siuiti [2] ; Alonso, Antonio [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Goias, Inst Fis, Goiania, Go - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: International Journal of Pharmaceutics; v. 532, n. 1, p. 547-554, OCT 30 2017.
Web of Science Citations: 3
Abstract

The sesquiterpene nerolidol and the monoterpene limonene are potent skin-permeation enhancers that have also been shown to have antitumor, antibacterial, antifungal and antiparasitic activities. Because terpenes are membrane-active compounds, we used electron paramagnetic resonance (EPR) spectroscopy of three membrane spin labels combined with the fluorescence spectroscopy of three lipid probes to study the interactions of these terpenes with stratum corneum (SC) intercellular membranes. An experimental apparatus was developed to assess the lipid fluidity of hydrated SC membranes via the fluorescence anisotropy of extrinsic membrane probes. Both EPR and fluorescence probes indicated that the intercellular membranes of neonatal SC rats undergo a main phase transition at approximately 50 degrees C. Taken together, the results indicated that treatment with 1% nerolidol (v/v) caused large fluidity increases in the more ordered phases of SC membranes and that these effects gradually decreased with increasing temperature. Additionally, compared with (+)-limonene, nerolidol was better able to change the SC membrane dynamics. EPR and fluorescence data suggest that these terpenes act as spacers in lipid packaging and create increased lipid disorder in the more ordered regions and phases of SC membranes, notably leading to a population of probes with less restricted motion. (AU)

FAPESP's process: 14/26895-7 - Heterogeneities in lipids and proteins structures: studies by advanced fluorescence techniques
Grantee:Amando Siuiti Ito
Support Opportunities: Regular Research Grants