| Full text | |
| Author(s): |
de Melo, Karina Mescouto
[1]
;
de Moraes-Pinto, Maria Isabel
[1]
;
Andrade, Luis E. C.
[2]
;
Salomao, Reinaldo
[2]
;
Brunialti, Milena K. C.
[2]
;
Ferreira, Vanessa S.
[2]
;
Costa-Carvalho, Beatriz T.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo, UNIFESP, Escola Paulista Med, Dept Pediat, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, UNIFESP, Escola Paulista Med, Dept Med, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | APMIS; v. 125, n. 10, p. 902-909, OCT 2017. |
| Web of Science Citations: | 1 |
| Abstract | |
This study investigated phenotypic and functional characteristics of lymphocytes in children with common variable immunodeficiency (CVID) and unclassified hypogammaglobulinemia (UH), as well as B-cell subsets in non-consanguineous parents. Blood samples of 30 children, CVID (n = 9), UH (n = 9), healthy donors HD (n = 12), and 19 adults (parents and controls) were labeled by a combination of surface markers to identify CD4, CD8 T-cell and B-cell sub-populations. T-cell cytokine production in children was analyzed in vitro after stimulation with phytohemagglutinin (PHA) and tetanus toxoid. We observed low percentages of switched memory B cells in children with CVID, increase in total CD4(+) T-cell counts, and high percentages of transitional B cells only in UH group. Analysis of T-cell immunity showed that CVID children had decreased percentages of CD8(+) IFN-gamma-producing cells after stimulation with PHA and tetanus toxoid. Parent of children with CVID had low percentages of naive B cell and increased percentages of memory B cells in comparison with controls. These results suggest that (i) early combined immune defect in children with CVID and (ii) a possible familial B-cell disturbance in pediatric CVID. (AU) | |
| FAPESP's process: | 10/20834-5 - Functional evaluation of B cell subsets in children with hypogammaglobulinemia |
| Grantee: | Beatriz Tavares Costa Carvalho |
| Support Opportunities: | Regular Research Grants |