| Full text | |
| Author(s): |
William Gemio Jacobsen Teixeira
[1]
;
Alexandre Fogaça Cristante
[2]
;
Raphael Martus Marcon
[3]
;
Gustavo Bispo
[4]
;
Ricardo Ferreira
[3]
;
Tarcísio Eloy Pessoa de Barros-Filho
[6]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, ICESP, Div Cirurgia Coluna, Tumores Coluna, Sao Paulo, SP - Brazil
[2] Universidade de São Paulo. Laboratorio de Investigacao Medica, Instituto de Ortopedia e Traumatologia (IOT), Hospital das Clinicas HCFMUSP, Faculdade de Medicina. Divisao de Cirurgia da Coluna - Brasil
[3] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Div Cirurgia Coluna, Lab Invest Med, IOT, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Hosp Clin HCFMUSP, IOT, Fac Med, LIM 41, Sao Paulo, SP - Brazil
[5] Universidade de São Paulo. Laboratorio de Investigacao Medica, Instituto de Ortopedia e Traumatologia (IOT), Hospital das Clinicas HCFMUSP, Faculdade de Medicina. Divisao de Cirurgia da Coluna - Brasil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Clinics; v. 73, 2018-02-19. |
| Abstract | |
OBJECTIVES: To evaluate the effects of combined treatment with granulocyte colony-stimulating factor (G-CSF) and methylprednisolone in rats subjected to experimental spinal cord injury. METHODS: Forty Wistar rats received a moderate spinal cord injury and were divided into four groups: control (no treatment); G-CSF (G-CSF at the time of injury and daily over the next five days); methylprednisolone (methylprednisolone for 24 h); and G-CSF/Methylprednisolone (methylprednisolone for 24 h and G-CSF at the time of injury and daily over the next five days). Functional evaluation was performed using the Basso, Beattie and Bresnahan score on days 2, 7, 14, 21, 28, 35 and 42 following injury. Motor-evoked potentials were evaluated. Histological examination of the spinal cord lesion was performed immediately after euthanasia on day 42. RESULTS: Eight animals were excluded (2 from each group) due to infection, a normal Basso, Beattie and Bresnahan score at their first evaluation, or autophagy, and 32 were evaluated. The combination of methylprednisolone and G-CSF promoted greater functional improvement than methylprednisolone or G-CSF alone (p<0.001). This combination also exhibited a synergistic effect, with improvements in hyperemia and cellular infiltration at the injury site (p<0.001). The groups displayed no neurophysiological differences (latency p=0.85; amplitude p=0.75). CONCLUSION: Methylprednisolone plus G-CSF promotes functional and histological improvements superior to those achieved by either of these drugs alone when treating spinal cord contusion injuries in rats. Combining the two drugs did have a synergistic effect. (AU) | |
| FAPESP's process: | 13/14230-8 - Study of the combined effect of granulocyte-colony stimulating factor with methylprednisolone in the treatment of acute spinal cord injury in rats |
| Grantee: | Alexandre Fogaça Cristante |
| Support Opportunities: | Regular Research Grants |