Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Electrostatic Self-Assembled Chitosan-Pectin Nano- and Microparticles for Insulin Delivery

Full text
Author(s):
Maciel, Vinicius B. V. [1, 2] ; Yoshida, Cristiana M. P. [3] ; Pereira, Susana M. S. S. [4] ; Goycoolea, Francisco M. [5, 4] ; Franco, Telma T. [1]
Total Authors: 5
Affiliation:
[1] UNICAMP State Univ Campinas, Sch Chem Engn, Av Albert Einstein 500, BR-13083852 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Anim Sci & Food Engn, Av Duque Caxias Norte 225, BR-13635900 Sao Paulo - Brazil
[3] UNIFESP Fed Univ Sao Paulo, Dept Exact & Earth Sci, Rua Sao Nicolau 210, BR-09913030 Sao Paulo - Brazil
[4] Westfalische Wilhelms Univ Munster, Inst Biol & Biotechnol Pflanzen, Schlossgarten 3, D-48149 Munster - Germany
[5] Univ Leeds, Sch Food Sci & Nutr, Leeds LS2 9JT, W Yorkshire - England
Total Affiliations: 5
Document type: Journal article
Source: Molecules; v. 22, n. 10 OCT 2017.
Web of Science Citations: 21
Abstract

A polyelectrolyte complex system of chitosan-pectin nano-and microparticles was developed to encapsulate the hormone insulin. The aim of this work was to obtain small particles for oral insulin delivery without chemical crosslinkers based on natural and biodegradable polysaccharides. The nano-and microparticles were developed using chitosans (with different degrees of acetylation: 15.0% and 28.8%) and pectin solutions at various charge ratios (n(+)/n(-) given by the chitosan/pectin mass ratio) and total charge. Nano-and microparticles were characterized regarding particle size, zeta potential, production yield, encapsulation efficiency, stability in different media, transmission electron microscopy and cytotoxicity assays using Caco-2 cells. The insulin release was evaluated in vitro in simulated gastric and intestinal media. Small-sized particles (similar to 240-similar to 1900 nm) with a maximum production yield of similar to 34.0% were obtained. The highest encapsulation efficiency (similar to 62.0%) of the system was observed at a charge ratio (n(+)/n(-)) 5.00. The system was stable in various media, particularly in simulated gastric fluid (pH 1.2). Transmission electron microscopy (TEM) analysis showed spherical shape particles when insulin was added to the system. In simulated intestinal fluid (pH 6.8), controlled insulin release occurred over 2 h. In vitro tests indicated that the proposed system presents potential as a drug delivery for oral administration of bioactive peptides. (AU)

FAPESP's process: 16/18142-4 - Biopolymer-based nano- and micro-particles loaded with a natural plant (Pereskia aculeata Miller) extract for delivery of iron.
Grantee:Vinícius Borges Vieira Maciel
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor