Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies

Author(s):Show less -

Rocha, Leticia Barboza ^[1] ; dos Santos Alves, Rubens Prince ^[2] ; Caetano, Bruna Alves ^[1] ; Pereira, Lennon Ramos ^[2] ; Mitsunari, Thais ^[1] ; Amorim, Jaime Henrique ^{[2, 3]} ; Polatto, Juliana Moutinho ^[1] ; Botosso, Viviane Fongaro ^[4] ; Frazatti Gallina, Neuza Maria ^{[5, 6]} ; Palacios, Ricardo ^[7] ; Precioso, Alexander Roberto ^[7] ; Hernandes Granato, Celso Francisco ^[8] ; Leal Oliveira, Danielle Bruna ^[9] ; da Silveira, Vanessa Barbosa ^[9] ; Luz, Daniela ^[1] ; de Souza Ferreira, Luis Carlos ^[2] ; Fontes Piazza, Roxane Maria ^[1]

Affiliation

^[1] Inst Butantan, Lab Bacteriol, BR-05503900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Lab Dev Vacinas, Inst Ciencias Biomed, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Fed Oeste Bahia, Ctr Ciencias Biol & Saude, Lab Microbiol, BR-47805100 Barreiras, Bahia - Brazil

^[4] Inst Butantan, Lab Virol, BR-05503900 Sao Paulo, SP - Brazil

^[5] Div Dev Tecnol & Prod, BR-05503 Sao Paulo, SP - Brazil

^[6] Inst Butantan, BR-05503 Sao Paulo, SP - Brazil

^[7] Inst Butantan, Div Ensaios Clin & Farmacovigilancia, BR-05503900 Sao Paulo, SP - Brazil

^[8] Univ Fed Sao Paulo, Dept Medicina, Disciplina Doencas Infecciosas & Parasitarias, BR-04023062 Sao Paulo, SP - Brazil

^[9] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Lab Virol Mol & Clin, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: ANTIBODIES; v. 6, n. 4 DEC 2017.
• Web of Science Citations: 4

Abstract

Dengue nonstructural protein 1 (NS1) is a multi-functional glycoprotein with essential functions both in viral replication and modulation of host innate immune responses. NS1 has been established as a good surrogate marker for infection. In the present study, we generated four anti-NS1 monoclonal antibodies against recombinant NS1 protein from dengue virus serotype 2 (DENV2), which were used to map three NS1 epitopes. The sequence (193)AVHADMGYWIESALNDT(209) was recognized by monoclonal antibodies 2H5 and 4H1BC, which also cross-reacted with Zika virus (ZIKV) protein. On the other hand, the sequence (25)VHTWTEQYKFQPES(38) was recognized by mAb 4F6 that did not cross react with ZIKV. Lastly, a previously unidentified DENV2 NS1-specific epitope, represented by the sequence (127)ELHNQTFLIDGPETAEC(143), is described in the present study after reaction with mAb 4H2, which also did not cross react with ZIKV. The selection and characterization of the epitope, specificity of anti-NS1 mAbs, may contribute to the development of diagnostic tools able to differentiate DENV and ZIKV infections. (AU)

• FAPESP's process: 13/06589-6 - Immunochromatographic assay as diagnostic tool for neglected diseases: studies of improvement, scheduling and feasibility
• Grantee: Roxane Maria Fontes Piazza
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/17595-0 - New vaccine research based on recombinant proteins of dengue virus
• Grantee: Luis Carlos de Souza Ferreira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/09096-8 - DIAGNOSIS INNOVATION USING IMMUNOCHROMATHOGRAPHIC TESTS: A TOOL FOR DETECTION OF NEGLECTED DISEASES
• Grantee: Letícia Barboza Rocha
• Support Opportunities: Scholarships in Brazil - Post-Doctoral