Phylogeny of Anophelinae using mitochondrial protein coding genes

Author(s):Show less -

Foster, Peter G. ^[1] ; Porangaba de Oliveira, Tatiane Marques ^[2] ; Bergo, Eduardo S. ^[3] ; Conn, Jan E. ^{[4, 5]} ; Sant'Ana, Denise Cristina ^[2] ; Nagaki, Sandra Sayuri ^[2] ; Nihei, Silvio ^[6] ; Lamas, Carlos Einicker ^[7] ; Gonzalez, Christian ^[8] ; Moreira, Caio Cesar ^[2] ; Mureb Sallum, Maria Anice ^[2]

Affiliation

^[1] Nat Hist Museum, Dept Life Sci, London - England

^[2] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01246904 Sao Paulo, SP - Brazil

^[3] Secretaria Estado Saude Sao Paulo, Superintendencia Controle Endemias, Sao Paulo, SP - Brazil

^[4] New York State Dept Hlth, Wadsworth Ctr, Albany, NY - USA

^[5] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Albany, NY - USA

^[6] Univ Sao Paulo, Inst Biociencias, BR-05508900 Sao Paulo, SP - Brazil

^[7] Univ Sao Paulo, Museu Zool, BR-04263000 Sao Paulo, SP - Brazil

^[8] Univ Metropolitana Ciencias Educ, Inst Entomol, Santiago - Chile

• Document type: Journal article
• Source: ROYAL SOCIETY OPEN SCIENCE; v. 4, n. 11 NOV 2017.
• Web of Science Citations: 18

Abstract

Malaria is a vector-borne disease that is a great burden on the poorest and most marginalized communities of the tropical and subtropical world. Approximately 41 species of Anopheline mosquitoes can effectively spread species of Plasmodium parasites that cause human malaria. Proposing a natural classification for the subfamily Anophelinae has been a continuous effort, addressed using both morphology and DNA sequence data. The monophyly of the genus Anopheles, and phylogenetic placement of the genus Bironella, subgenera Kerteszia, Lophopodomyia and Stethomyia within the subfamily Anophelinae, remain in question. To understand the classification of Anophelinae, we inferred the phylogeny of all three genera (Anopheles, Bironella, Chagasia) and major subgenera by analysing the amino acid sequences of the 13 protein coding genes of 150 newly sequenced mitochondrial genomes of Anophelinae and 18 newly sequenced Culex species as outgroup taxa, supplemented with 23 mitogenomes from GenBank. Our analyses generally place genus Bironella within the genus Anopheles, which implies that the latter as it is currently defined is not monophyletic. With some inconsistencies, Bironella was placed within the major clade that includes Anopheles, Cellia, Kerteszia, Lophopodomyia, Nyssorhynchus and Stethomyia, which were found to be monophyletic groups within Anophelinae. Our findings provided robust evidence for elevating the monophyletic groupings Kerteszia, Lophopodomyia, Nyssorhynchus and Stethomyia to genus level; genus Anopheles to include subgenera Anopheles, Baimaia, Cellia and Christya; Anopheles parvus to be placed into a new genus; Nyssorhynchus to be elevated to genus level; the genus Nyssorhynchus to include subgenera Myzorhynchella and Nyssorhynchus; Anopheles atacamensis and Anopheles pictipennis to be transferred from subgenus Nyssorhynchus to subgenus Myzorhynchella; and subgenus Nyssorhynchus to encompass the remaining species of Argyritarsis and Albimanus Sections. (AU)

• FAPESP's process: 14/26229-7 - Latitudinal landscape genomics and ecology of Anopheles darlingi
• Grantee: Maria Anice Mureb Sallum
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 11/20397-7 - Morphological and molecular taxonomy and phylogeny of Nyssorhynchus subgenus of Anopheles (Diptera: Culicidae), with special emphasis on Anopheles darlingi from Mata Atlântica
• Grantee: Maria Anice Mureb Sallum
• Support type: Regular Research Grants