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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Estrogen receptor 1 (ESR1) regulates VEGFA in adipose tissue

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Fatima, L. A. [1, 2] ; Campello, R. S. [1] ; Santos, R. de Souza [2] ; Freitas, H. S. [1] ; Frank, A. P. [2] ; Machado, U. F. [1] ; Clegg, D. J. [2]
Total Authors: 7
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
[2] Cedars Sinai Med Ctr, Diabet & Obes Res Div, Biomed Res Dept, Los Angeles, CA 90048 - USA
Total Affiliations: 2
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 7, DEC 1 2017.
Web of Science Citations: 7

Vascular endothelial growth factor A (VEGFA) is a key factor in the regulation of angiogenesis in adipose tissue. Poor vascularization during adipose tissue proliferation causes fibrosis and local inflammation, and is associated with insulin resistance. It is known that 17-beta estradiol (E2) regulates adipose tissue function and VEGFA expression in other tissues; however, the ability of E2 to regulate VEGFA in adipose tissue is currently unknown. In this study, we showed that, in 3T3-L1 cells, E2 and the estrogen receptor 1 (ESR1) agonist PPT induced VEGFA expression, while ESR1 antagonist (MPP), and selective knockdown of ESR1 using siRNA decreased VEGFA and prevented the ability of E2 to modulate its expression. Additionally, we found that E2 and PPT induced the binding of hypoxia inducible factor 1 alpha subunit (HIF1A) in the VEGFA gene promoter. We further found that VEGFA expression was lower in inguinal and gonadal white adipose tissues of ESR1 total body knockout female mice compared to wild type mice. In conclusion, our data provide evidence of an important role for E2/ESR1 in modulating adipose tissue VEGFA, which is potentially important to enhance angiogenesis, reduce inflammation and improve adipose tissue function. (AU)

FAPESP's process: 13/03343-6 - VEGFA-mediated role of estradiol on angiogenesis/adipogenesis process in the adipose tissue
Grantee:Luciana Alves de Fátima
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/13652-1 - Effect of adipocyte knockdown of Esr1 gene on the regulation of VEGFA and Slc2a4/GLUT4 expression.
Grantee:Luciana Alves de Fátima
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 12/04831-1 - New players in glycemic control and chronic complications of Diabetes mellitus: preventive and therapeutic perspectives
Grantee:Ubiratan Fabres Machado
Support type: Research Projects - Thematic Grants