Selective and simultaneous determination of indigo carmine and allura red in candy samples at the nano-concentration range by flow injection analysis with multiple pulse amperometric detection

Author(s)

Deroco, Patricia B. ^[1] ; Medeiros, Roberta A. ^[2] ; Rocha-Filho, Romeu C. ^[1] ; Fatibello-Filho, Orlando ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Quim, CP 676, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Estadual Londrina, Dept Quim, CP 6001, BR-86051990 Londrina, PR - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 247, p. 66-72, MAY 1 2018.
• Web of Science Citations: 10

Abstract

A novel, unique electroanalytical method was developed for the simultaneous quantification of the dyes indigo carmine (IC) and allura red (AR) in candies by coupling flow injection analysis and multiple pulse amperometry with a cathodically pretreated boron-doped diamond electrode, using 0.30 mol L-1 H2SO4 as supporting electrolyte. A dual-potential waveform was employed, causing the electrooxidation of either IC solely or IC and AR simultaneously. Thence, subtraction of current signals was used to quantify IC and AR in the concentration ranges of 70.0-1000 nmol L-1 and 40.0-770 nmol L-1, with limits of detection of 40.0 nmol L-1 and 7.0 nmol L-1, respectively. The proposed method, which permits up to 153 determinations per hour with good precision, was successfully applied in the quantification of these dyes in samples of commercial candies; their obtained contents were similar (at a 95% confidence level) to those from a comparative HPLC method. (AU)

• FAPESP's process: 14/07919-2 - Preparation of electrodes with new carbon-based materials for applications in electroanalytical
• Grantee: Patrícia Batista Deroco
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/08180-5 - DEVELOPMENT OF ELECTROANALYTICAL METHODS EMPLOYING A BORON-DOPED DIAMOND ELECTRODE FOR SIMULTANEOUS DETERMINATION OF SWEETENERS AND COLORANTS IN FOOD SAMPLES
• Grantee: Patrícia Batista Deroco
• Support Opportunities: Scholarships in Brazil - Master