Cocoplum (Chrysobalanus icaco L.) decreases doxorubicin-induced DNA damage and downregulates Gadd45a, Il-1 beta, and Tnf-alpha in vivo

Author(s)

Venancio, Vinicius Paula ^{[1, 2]} ; Almeida, Mara Ribeiro ^[1] ; Greggi Antunes, Lusania Maria ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 - USA

• Document type: Journal article
• Source: Food Research International; v. 105, p. 996-1002, MAR 2018.
• Web of Science Citations: 2

Abstract

DNA damage and inflammation are promising targets in disease prevention studies. Since these pathways have shown to be modulated by dietary components, investigating the molecular effects of food becomes relevant. This study aimed at investigating the protective effects of cocoplum (Chrysobalanus icaco L.) against doxorubicin (DXR)-induced damage. Rats were treated with cocoplum (100, 200 or 400 mg/kg/day) for 14 days, associated or not with DXR (15 mg/kg b.w.). Tissue-targeted comet assay and the oxidative stress parameters oxidized/reduced glutathione and catalase were investigated in liver, kidney, and heart. The expressions of DNA damage/repair (Gadd45a, Parpl, Xrcc2) and proinflammatory genes (Il-1 beta,Il-6, Nf-kappa b, Trtf-alpha) were performed by real-time quantitative PCR. Cocoplum decreased DNA damage and the expressions of Gadd45a, Il-1 beta, and Thf-alpha induced by DXR. These findings demonstrate that cocoplum fruits possess antigenotoxic and anti-inflammatory effects against DXR-induced damage and encourage other in vivo/clinical studies with this fruit. (AU)

• FAPESP's process: 11/21471-6 - In vitro and in vivo activities of guajiru fruit (Chrysobalanus icaco L.) in oxidative stress, DNA damage and inflammation biomarkers
• Grantee: Vinicius de Paula Venancio
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)