Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro

Author(s):Show less -

Ribeiro, Diego Luis ^[1] ; Ciliao, Heloisa Lizotti ^[1] ; Leal Specian, Ana Flavia ^[1] ; Serpeloni, Juliana Mara ^{[1, 2]} ; De Oliveira, Marcelo Tempesta ^[1] ; Varanda, Eliana Aparecida ^[2] ; Vilegas, Wagner ^[3] ; Saldanha, Luiz Leonardo ^{[4, 5]} ; Martinez-Lopez, Wilner ^[6] ; Dokkedal, Anne Ligia ^[4] ; Syllos Colus, Ilce Mara ^[1]

Affiliation

^[1] State Univ Londrina UEL, Ctr Biol Sci, Dept Gen Biol, Rod Celso Garcia Cid PR 445, Km 380, Univ Campus, BR-86057970 Londrina, PR - Brazil

^[2] So Paulo State Univ UNESP, Fac Pharmaceut Sci Araraquara, Dept Biol Sci, Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Campus Litoral Paulista, Sao Vicente, SP - Brazil

^[4] So Paulo State Univ UNESP, Biosci Inst, Dept Bot, Botucatu, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Fac Sci Bauru, Dept Biol Sci, Bauru, SP - Brazil

^[6] Inst Biol Invest Clemente Estable, Montevideo - Uruguay

• Document type: Journal article
• Source: Cytotechnology; v. 70, n. 2, p. 713-728, APR 2018.
• Web of Science Citations: 1

Abstract

Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla extract. The phytochemical profile highlight the presence of flavonoids isorhamentin and quercetin derivates. The MTT assay was used to evaluate the cytotoxicity of different concentrations for different treatment times. Three concentrations (7.5, 15, 30 A mu g/mL) were chosen for assessment of apoptosis (AO/EB), mutagenicity (micronucleus), and cell cycle kinetics (flow cytometry). Thereafter, the concentration of 7.5 A mu g/mL was chosen to evaluate the protective effects against DNA damage induced by benzo{[}a]pyrene (B{[}a]P). At concentrations higher than 7.5 A mu g/mL (between 10 and 50 A mu g/mL), the extract was cytotoxic, induced apoptosis, and caused antiproliferative effects. However, it did not induce micronucleus and a reduction of apoptotic and micronucleated cells was observed in treatments that included the extract and B{[}a]P. The protective effect is attributable to the presence of flavonoids, described as antioxidants, inhibitors of DNA adduct and activators of detoxifying enzymes. The results of the present study such as absence of cytotoxic and mutagenic effects and protective effects against known carcinogens suggest that B. holophylla has potential for use soon as herbal medicine. (AU)

• FAPESP's process: 12/01996-0 - Standardized phytoterapics for the treatment of chronic diseases: Cytotoxic, mutagenic and protective properties and the modulation of gene expression
• Grantee: Juliana Mara Serpeloni
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases
• Grantee: Wagner Vilegas
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 09/16147-5 - Chemical and pharmacological contribution to the Machaerium genera (Fabaceae)
• Grantee: Miriam Sannomiya
• Support Opportunities: Regular Research Grants