Comparison of the effect of root surface modification with citric acid, EDTA, and aPDT on adhesion and proliferation of human gingival fibroblasts and osteoblasts: an in vitro study

Author(s)

Ferreira, Rafael ^[1] ; de Toledo Barros, Renato Taddei ^[1] ; Brandao Hage Karam, Paula Stephania ^[1] ; Passanezi Sant'Ana, Adriana Campos ^[1] ; Aguiar Greghi, Sebastiao Luiz ^[1] ; Rubo de Rezende, Maria Lucia ^[1] ; Ragghianti Zangrando, Mariana Schutzer ^[1] ; de Oliveira, Rodrigo Cardoso ^[2] ; Damante, Carla Andreotti ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodont, Div Periodont, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodont, Div Biochem, Bauru, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 33, n. 3, p. 533-538, APR 2018.
• Web of Science Citations: 3

Abstract

Root demineralization is used in Periodontics as an adjuvant for mechanical treatment. The aim of this study was to evaluate the effects of root surface modification with mechanic, chemical, and photodynamic treatments on adhesion and proliferation of human gingival fibroblasts and osteoblasts. Root fragments were treated by scaling and root planing (C-control group), EDTA (pH 7), citric acid plus tetracycline (CA-pH 1), and antimicrobial photodynamic therapy (aPDT) with toluidine blue O and red laser (pH 4). Cells were seeded (104 cells/well, 6th passage) on root fragments of each experimental group and cultured for 24, 48, and 72 h. Cells were counted in scanning electron microscopy images by a calibrated examiner. For fibroblasts, the highest number of cells were present at 72-h period (p < 0.05). EDTA group showed a very low number of cells in relation to CA group (p < 0.05). CA and aPDT group presented higher number of cells in all periods, but without differences between other treatment groups (p > 0.05). For osteoblasts, there was a significant increase in cell numbers for aPDT group at 72 h (p < 0.05). In conclusion, aPDT treatment provided a positive stimulus to osteoblast growth, while for fibroblasts, aPDT and CA had a tendency for higher cell growth. (AU)

• FAPESP's process: 10/15667-2 - Effect of laser and photodynamic therapy on metallic implants surface decontamination: microscopic study in rats subcutaneous tissue
• Grantee: Maria Lúcia Rubo de Rezende
• Support type: Regular Research Grants
• FAPESP's process: 12/06738-9 - Evaluation of materials, instruments and protocols of irrigation used in Endodontic treatment, by means of scanning electron microscopy and micro analysis of x-ray.
• Grantee: Marco Antonio Hungaro Duarte
• Support type: Regular Research Grants