Proteomic analysis and cell morphology structuring of human gingival fibroblasts cultured on different dental implant surfaces

• Grant number: 11/16317-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: December 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Altair Antoninha Del Bel Cury
• Grantee: Cindy Goes Dodo
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Despite the proven success of rehabilitation with implants, there is a search for the surface treatment of titanium in order to improve the osseointegration process, especially for application in bone sites of low quality and immediate loading protocols. As this modification can be done by different processes, the treatment chosen and executed by each manufacturer may be reflected on surfaces with different chemical compositions, which is important in the adhesion and cell proliferation. Despite the use of this land is the common occurrence of cervical bone resorption, also called bone saucerization, which turns out to expose the cervical region of the implant to the subepithelial connective tissue of the peri-implant tissue. Because dental implants require a biological seal sponsored by the peri-implant tissue, functioning as a barrier to the movement of bacteria and toxins from the oral cavity to implant interface for the purpose of this study is to assess the influence of different surfaces of dental implants available in dental market in the process of cell adhesion and proliferation. For this, human gingival fibroblasts are cultured on dental implants with the following areas: 1. microtexture by the process of abrasive blasting and subtracting acid, 2. microtexture by anodization process, 3.microtextura with hydrophilic surface, 4. nanotextura, 5. microtexture with nanosized crystals of calcium phosphate and without surface treatment. The influence of each surface in the expression of proteins related to the process of adhesion and cell proliferation will be assessed by mass spectrometry (LC-MS/MS) of the cellular proteome in periods of 2, 7 and 14 days. In these same periods, the cells will be stained for visualization of three-dimensional morphology by confocal microscopy laser scanning. (AU)