Impaired Treg and NK cells profile in overweight women with gestational diabetes mellitus

Author(s)

Lobo, Thalita Frutuoso ^[1] ; Borges, Cannila de Moraes ^[1] ; Mattar, Rosiane ^[1] ; Gomes, Caio Perez ^[1] ; Santos de Angelo, Ana Geisa ^[1] ; Tezotto Pendeloski, Karen Priscilla ^[1] ; Daher, Silvia ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Obstet, Sao Paulo - Brazil

• Document type: Journal article
• Source: American Journal of Reproductive Immunology; v. 79, n. 3 MAR 2018.
• Web of Science Citations: 6

Abstract

Problem: Maternal obesity is frequently associated with gestational diabetes mellitus (GDM), and immunological mechanisms seem to be involved in the physiopathology of these conditions. The aim of this study was to characterize the profile of immune cells in peripheral blood of overweight women with GDM. Method of Study: This case-control study included 27 glucose-tolerant (controls) and 31 GDM overweight pregnant women. Flow cytometry was used to assess the number of regulatory T cells (Treg) and natural killer (NK) cells in the peripheral blood. In addition, the expression of IL-10, TGF-B, and TNF-A in Treg and expression of IFN-G, TNF-A, granzyme, and perforin in NK cells were analyzed. Results: GDM patients had significantly lower frequency of TCD4(+)CD25(bright) and TCD4(+)CD25(+)FOXP3(high) cells, higher production of TNF-A by Treg cells and higher percentage of NKCD16(+)56(dim) cells than the controls. Conclusion: The association between obesity and GDM is a condition where it is observed impaired Treg and NK cells profile, findings that seem to be related with the development of IR and inflammation. (AU)

• FAPESP's process: 13/22754-7 - Evaluation of immune cells profile in patients with gestational diabetes
• Grantee: Silvia Daher
• Support Opportunities: Regular Research Grants