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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dysregulated mitogen-activated protein kinase and matrix metalloproteinase in ethanol-induced cavernosal dysfunction

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Author(s):
Muniz, Jaqueline J. [1] ; Leite, Leticia N. [2, 1] ; Lacchini, Riccardo [1] ; Tanus-Santos, Jose E. [2] ; Tirapelli, Carlos R. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, DEPCH, Escola Enfermagem Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Farmacol, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Canadian Journal of Physiology and Pharmacology; v. 96, n. 3 MAR 2018.
Web of Science Citations: 1
Abstract

We evaluated the effects of ethanol consumption on the mitogen-activated protein kinases (MAPK) and metalloproteinases (MMP) pathways in the rat cavernosal smooth muscle (CSM). Male Wistar rats were treated with ethanol (20% v/v) for 6 weeks. Quantitative real-time polymerase chain reaction experiments showed that ethanol consumption did not alter mRNA levels of p38MAPK, SAPK/JNK, ERK1/2, MMP-2, or MMP-9 in the rat CSM. Western immunoblotting experiments revealed decreased protein expression of p38MAPK and phosphorylation of SAPK/JNK in the CSM from ethanol-treated rats. Additionally, ethanol consumption decreased the expression of MMP-2. Functional assays showed that SP600125, an inhibitor of SAPK/JNK, prevented the increase in endothelin (ET)-1-induced contraction in the CSM from ethanol-treated rats. Treatment with ethanol decreased MMP-2 activity, but did not change net MMP activity in the rat CSM. Ethanol consumption increased the circulating levels of MMP-2, MMP-9, and TIMP-2 as well as the MMP-9/TIMP-1 ratio. The major finding of our study is that ethanol consumption down-regulates both MAPK and MMP pathways in the rat CSM, whereas it increases the circulating levels of MMP-9. Additionally, we found that SAPK/JNK plays a role in ethanol-induced increase on ET-1 contraction in the isolated rat CSM. (AU)

FAPESP's process: 12/04144-4 - Consequences of chronic ethanol consumption on metalloproteinases and MAPKs pathways and tissue remodeling in the corpus cavernosum of rats.
Grantee:Jaqueline Jóice Muniz
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/12911-2 - Consequences of chronic ethanol consumption on the reactivity and expression of components of the endothelinergic system in the rat corpus cavernosum
Grantee:Carlos Renato Tirapelli
Support Opportunities: Regular Research Grants