Grooves surrounding the micropyle decrease the inseminating dose in fish

Author(s)

Pereira-Santos, Matheus ^[1] ; Shimoda, Eduardo ^[2] ; Cesar de Andrade, Andre Furugen ^[3] ; Silva, Luciano Andrade ^[3] ; Fujimoto, Takafumi ^[4] ; Senhorini, Jose Augusto ^[5] ; Yasui, George Shigueki ^[3] ; Okada Nakaghi, Laura Satiko ^[1]

Affiliation

^[1] Sao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[2] Univ Candido Mendes, Dept Pharm, Rua Anita Pecanha 100, BR-28030335 Campos Dos Goytacazes, RJ - Brazil

^[3] Univ Sao Paulo, Dept Vet, Ave Duque Caxias Norte 225, BR-13630080 Pirassununga, SP - Brazil

^[4] Hokkaido Univ, Fac Fisheries Sci, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611 - Japan

^[5] Natl Ctr Res & Conservat Continental Fish, Chico Mendes Inst Biodivers Conservat, Rodovia Pref Euberto Nemesio Pereira de Godoy, BR-13630970 Pirassununga, SP - Brazil

• Document type: Journal article
• Source: ZYGOTE; v. 25, n. 6, p. 731-739, DEC 2017.
• Web of Science Citations: 1

Abstract

In fish with external fertilization, sperm must reach the oocyte through the micropyle to enter the cytoplasm. Fertilization success is then influenced by characteristics of oocytes or sperm. In this study, we evaluated oocyte morphology and sperm motility parameters and their effects on the inseminating dose in a teleost fish Astyanax altiparanae. Interestingly, we found one of the lowest yet described inseminating doses in teleosts (2390 spermatozoa oocyte(-1) ml(-1)). Such a fertilization efficacy may be explained by the long duration of sperm motility (>75 s), the small oocyte diameter (695.119 mu m), large micropyle diameter (7.57 mu m), and the presence of grooves on the oocyte surface that guides spermatozoon to the fertilization area. Additionally, we have described for the first time a structure that combines grooves on the chorion surface and a ridge in the micropylar area. (AU)

• FAPESP's process: 12/18961-4 - Reproductive aspects, morphology of gametes and embryonic development of Astyanax altiparanae Garutti & Britski (Teleostei, Characidae)
• Grantee: Matheus Pereira dos Santos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 10/17429-1 - Chimerism in native fish species as a tool for surrogate propagation in aquaculture
• Grantee: George Shigueki Yasui
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 11/11664-1 - Chimerism in native fish species as a tool for surrogate propagation in aquaculture
• Grantee: George Shigueki Yasui
• Support Opportunities: Scholarships in Brazil - Young Researchers