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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of a novel sugar transporter involved in sugarcane bagasse degradation in &ITTrichoderma reesei&IT

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Author(s):
Nogueira, Karoline M. V. [1] ; de Paula, Renato Graciano [1] ; Campos Antonieto, Amanda Cristina [1] ; dos Reis, Thaila F. [2] ; Carraro, Claudia Batista [1] ; Silva, Alinne Costa [1] ; Almeida, Fausto [1] ; Vargas Rechia, Carem Gledes [2] ; Goldman, Gustavo H. [2] ; Silva, Roberto N. [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BIOTECHNOLOGY FOR BIOFUELS; v. 11, APR 2 2018.
Web of Science Citations: 4
Abstract

Background: Trichoderma reesei is a saprophytic fungus implicated in the degradation of polysaccharides present in the cell wall of plants. T. reesei has been recognized as the most important industrial fungus that secretes and produces cellulase enzymes that are employed in the production of second generation bioethanol. A few of the molecular mechanisms involved in the process of biomass deconstruction by T. reesei; in particular, the effect of sugar transporters and induction of xylanases and cellulases expression are yet to be known.\& para;\& para;Results: In our study, we characterized a novel sugar transporter, which was previously identified by our group through in silico analysis of RNA-seq data. The novel T. reesei 69957-sugar transport system (Tr69957) is capable of transporting xylose, mannose, and cellobiose using a T. reesei 69957-sugar transport system in Saccharomyces cerevisloe. The deletion of Tr69957 in T. reesei affected the fungal growth and biomass accumulation, and the sugar uptake in the presence of mannose, cellobiose, and xylose. Molecular docking studies revealed that Tr69957 shows reduced protein-ligand binding energy for interactions towards disaccharides in comparison with monosaccharides. Furthermore, the deletion of Tr69957 affected the gene expression of cellobiohydrolases (cel 7a and cel6a), beta-glucosidases (cel3a and cell a), and xylanases (xyn 1 and xyn2) in the cultures of parental and mutant strains in the presence of cellobiose and sugarcane bagasse (SCB).\& para;\& para;Conclusion: The transporter Tr69957 of T. reesei can transport cellobiose, xylose, and mannose, and can affect the expression of a few genes encoding enzymes, such as cellulases and xylanases, in the presence of SCB. We showed for the first time that a filamentous fungus (T. reesei) contains a potential mannose transporter that may be involved in the degradation of cellulose. (AU)

FAPESP's process: 15/09553-8 - Characterization of new sugar transporters involved in the regulation of lignocellulosic biomass degradation in Trichoderma reesei
Grantee:Karoline Maria Vieira Nogueira
Support Opportunities: Scholarships in Brazil - Doctorate