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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of superstimulation on the expression of microRNAs and genes involved in steroidogenesis and ovulation in Nelore cows

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Author(s):
Santos, P. H. [1] ; Satrapa, R. A. [2] ; Fontes, P. K. [1] ; Franchi, F. F. [1] ; Razza, E. M. [1] ; Mani, F. [3] ; Nogueira, M. F. G. [4] ; Barros, C. M. [1] ; Castilho, A. C. S. [4]
Total Authors: 9
Affiliation:
[1] Univ Estadual Paulista, UNESP, Inst Biosci, Dept Pharmacol, Campus Botucatu, Botucatu, SP - Brazil
[2] Univ Fed Acre UFAC, Ctr Biol & Nat Sci, Rio Branco, Acre - Brazil
[3] Univ Estadual Paulista, UNESP, Inst Biosci, Dept Chem & Biochem, Campus Botucatu, Botucatu, SP - Brazil
[4] Univ Estadual Paulista, Sch Sci & Languages, UNESP, Dept Biol Sci, Campus Assis, Assis, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Theriogenology; v. 110, p. 192-200, APR 1 2018.
Web of Science Citations: 3
Abstract

To better understand the impact of ovarian superstimulation on bovine follicular microenvironment, Nelore cows (Bos taurus indicus) were subjected to ovarian superstimulation with follicle stimulating hormone (FSH, n = 10; P-36 protocol) or FSH combined with eCG (n = 10; P-36/eCG protocol). Follicular fluid was analyzed for cholesterol concentration. Granulosa cells were analyzed by RT-qPCR to assess the expression of genes involved in steroidogenic and ovulatory and expression of microRNAs involved in final follicular development and luteinizing hormone/choriogonadotropin receptor (LHCGR) expression. Plasma concentration of estradiol was also measured. Follicular fluid from the P-36 group showed higher concentration of cholesterol than that of control (non-superstimulated) cows. Plasma concentration of estradiol was higher in the P-36/eCG group. Abundance of STAR and FSHR mRNAs were lower in granulosa cells from the P-36/eCG group. In contrast, LHCGR mRNA abundance was higher in superstimulated granulosa cells from the P-36 group and showed a pattern opposite to that of miR-222 expression. Ovarian superstimulation did not affect the expression of other markers (mmu-miR-202-5p, has-miR-873, has-miR-144, and their target genes, CREB, TGFBR2, and ATG7) of antral follicle development. However, the mRNA expression of VEGF pathway components was modulated by P-36 treatment. Taken together, these results demonstrate that superstimulatory protocols modify steroidogenic capacity, increase plasma estradiol, and regulate the abundance of VEGF system, LHCGR mRNA and suppress the expression of miR-222 in bovine granulosa cells. (C) 2017 Published by Elsevier Inc. (AU)

FAPESP's process: 13/11480-3 - Characterization of microRNAs (miRNAs) in bovine antral follicles from Nelore cattle: involvement during ovarian follicle cells differentiation and regulation of LH receptor (LHr) expression
Grantee:Anthony César de Souza Castilho
Support type: Research Grants - Young Investigators Grants