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Characterization of microRNAs (miRNAs) in bovine antral follicles from Nelore cows: involvement during ovarian follicle cells differentiation and regulation of LH receptor (LHR) expression

Grant number: 15/04505-5
Support type:Multi-user Equipment Program
Duration: June 01, 2015 - May 31, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:Anthony César de Souza Castilho
Grantee:Anthony César de Souza Castilho
Home Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
Associated research grant:13/11480-3 - Characterization of microRNAs (miRNAs) in bovine antral follicles from Nelore cattle: involvement during ovarian follicle cells differentiation and regulation of LH receptor (LHr) expression, AP.JP
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Type of equipment:Caracterização e Análises de Amostras - Proteínas/Ácidos nuclêicos - Arrays
Caracterização e Análises de Amostras - Proteínas/Ácidos nuclêicos - Sequenciadores
Manufacturer: Fabricante não informado
Model: Modelo não informado


Luteinizing hormone (LH) plays a key role in the control of physiological processes such as follicle development, ovulation and luteal maintenance in the ovary acting via, LHR. The LHR expression in bovine granulosa cells is crucial for the transition of FSH/LH-dependency in antral follicles. In cattle during the growth and differentiation of granulosa cells, there is an increase on LHR mRNA abundance. Among several factors that control the ovarian development, there is an involvement of microRNAs (miRNAs). MiRNAs are small no-coding RNA molecules composed by 19-25 base pairs that control the cellular translation. Studies have characterized the presence and dual actions of miRNAs in mammalian female reproductive tract, including, deleterious effects on female fertility, as the reduction in ovulatory capacity. Inversely, there is a pro-follicular effect, as inhibition of apoptosis in ovarian follicular by in vivo administration of miRNAs. Recent data has showed miRNAs that regulate LHR expression in rodents. In cattle, some studies have identified the profile of miRNAs expressed in adult and fetal ovaries and characterized the regulatory effect of miRNAs on mRNA targets involved in ovarian follicular differentiation, but not in the context aimed in this work. Thus, this project using four experiments, aims to investigate and characterize the profile of miRNAs expressed in granulosa cells during follicle deviation in Nelore heifers and controller of LHR mRNA expression, as well as its regulation by pro-survival factors for follicles (IGF1) and gonadotropins (FSH and eCG) used in pharmacological strategies for control of the estrous cycle in cattle. Additionally, we intend to evaluate in vitro actions of miRNAs on the regulation of LHR expression and consequently on the steroidogenesis of granulosa cells. Therefore, it is expected that the compilation of the results generated by this study may be identify new targets for enhanced reproductive efficiency in cattle different pharmacological on strategies for the manipulation of the estrus cycle, producing a "molecular signature" of miRNAs required for the development of the follicle dominant in Nellore females, stimulating, perhaps, the development of molecular therapies through the use of miRNAs (in situ) to post transcriptional regulation in the bovine ovary in females of low fertility. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FRANCHI, FERNANDA FAGALI; HERNANDES, MARIANA PELICHO; COALHO FERREIRA, AMANDA LETICIA; VIEIRA DE LIMA, VICTOR AUGUSTO; MENDES, LEONARDO DE OLIVEIRA; DE AQUINO, ARIANA MUSA; SCARANO, WELLERSON RODRIGO; DE SOUZA CASTILHO, ANTHONY CESAR. Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary. Biochemical and Biophysical Research Communications, v. 523, n. 4, p. 823-828, MAR 19 2020. Web of Science Citations: 0.
GIROTO, ALAN B.; FONTES, PATRICIA K.; FRANCHI, FERNANDA F.; DOS SANTOS, PRISCILA H.; RAZZA, EDUARDO M.; NOGUEIRA, MARCELO F. G.; MAIOLI, MARCOS A.; NOGUEIRA, GUILHERME P.; NUNES, GIOVANA B.; MINGOTI, GISELE Z.; MARECO, EDSON A.; CASTILHO, ANTHONY C. S. Use of pregnancy-associated plasma protein-A during oocyte in vitro maturation increases IGF-1 and affects the transcriptional profile of cumulus cells and embryos from Nelore cows. Molecular Reproduction and Development, v. 86, n. 11, p. 1694-1704, NOV 2019. Web of Science Citations: 0.
PACINI, ENIO S. A.; CASTILHO, NTHONY C. S.; HEBELER-BARBOSA, FLAVIA; PUPO, ANDRE S.; KIGUTI, LUIZ R. A. Contraction of Rat Cauda Epididymis Smooth Muscle to alpha(1)-Adrenoceptor Activation Is Mediated by alpha(1A)-Adrenoceptors. Journal of Pharmacology and Experimental Therapeutics, v. 366, n. 1, p. 21-28, JUL 1 2018. Web of Science Citations: 3.

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