| Full text | |
| Author(s): |
Nogueira, Marcelo Saito
[1]
;
Cosci, Alessandro
[1]
;
Teixeira Rosa, Ramon Gabriel
[1]
;
Salvio, Ana Gabriela
[2]
;
Pratavieira, Sebastiao
[1]
;
Kurachi, Cristina
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil
[2] Amaral Carvalho Hosp, Jahu, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of Biomedical Optics; v. 22, n. 12 DEC 2017. |
| Web of Science Citations: | 5 |
| Abstract | |
Fluorescence spectroscopy and lifetime techniques are potential methods for optical diagnosis and characterization of biological tissues with an in-situ, fast, and noninvasive interrogation. Several diseases may be diagnosed due to differences in the fluorescence spectra of targeted fluorophores, when, these spectra are similar, considering steady-state fluorescence, others may be detected by monitoring their fluorescence lifetime. Despite this complementarity, most of the current fluorescence lifetime systems are not robust and portable, and not being feasible for clinical applications. We describe the assembly of a fluorescence lifetime spectroscopy system in a suitcase, its characterization, and validation with clinical measurements of skin lesions. The assembled system is all encased and robust, maintaining its mechanical, electrical, and optical stability during transportation, and is feasible for clinical measurements. The instrument response function measured was about 300 ps, and the system is properly calibrated. At the clinical study, the system showed to be reliable, and the achieved spectroscopy results support its potential use as an auxiliary tool for skin diagnostics. (c) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE) (AU) | |
| FAPESP's process: | 13/07276-1 - CEPOF - Optics and Photonic Research Center |
| Grantee: | Vanderlei Salvador Bagnato |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 14/16154-0 - Fluorescence spectroscopy for diagnosis of clinically similar skin lesions |
| Grantee: | Marcelo Saito Nogueira |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences |
| Grantee: | Vanderlei Salvador Bagnato |
| Support Opportunities: | Research Projects - Thematic Grants |