IgG from atopic dermatitis patients induces IL-17 and IL-10 production in infant intrathymic TCD4 and TCD8 cells

Author(s):Show less -

Sgnotto, Fabio D. R. ^[1] ; de Oliveira, Marilia G. ^[1] ; Lira, Aline A. L. ^[1] ; Inoue, Amanda H. S. ^[1] ; Titz, Tiago O. ^[1] ; Orfali, Raquel L. ^[1] ; Bento-de-Souza, Luciana ^[1] ; Sato, Maria N. ^[1] ; Aoki, Valeria ^[1] ; Duarte, Alberto J. S. ^{[1, 2]} ; Victor, Jefferson R. ^{[1, 2, 3]}

Affiliation

^[1] Univ Sao Paulo, Lab Med Invest LIM 56, Div Dermatol, Sch Med, Ave Dr Eneas Carvalho Aguiar 500, 3rd Floor, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Div Pathol, Sch Med, Sao Paulo - Brazil

^[3] Laureate Int Univ, Div Environm Hlth, FMU, Sao Paulo - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF DERMATOLOGY; v. 57, n. 4, p. 434-440, APR 2018.
• Web of Science Citations: 5

Abstract

IntroductionOur group recently demonstrated that IgG modulates T cell cytokine production during the maturation process in the human thymus. The effects of this modulation are IgG repertoire dependent and can exert a systemic and long-term impact. ObjectiveTo investigate whether IgG from atopic dermatitis (AD) patients can modulate cytokine production of infant intrathymic TCD4 and TCD8 cells in vitro. MethodsThymic tissues were obtained from newborn children from nonatopic mothers, and thymocytes were cultured for 6 days with purified IgG from AD patients or with intravenous immunoglobulin (IVIG) or mock conditions as controls. Cells were gated as double positive T cells (TDP(-)CD4(+)CD8(+)), TCD4 cells (CD4(+)CD8(-)), or TCD8 cells (CD4(-)CD8(+)), and intracellular levels of IL-17A, IFN-, TNF-, IL-4, IL-10, and TGF- were evaluated by flow cytometry. ResultsCompared to mock and IVIG culture conditions, IgG of AD individuals induced in vitro intracellular production of IL-17 and IL-10 by intrathymic TDP, TCD4, and TCD8 cells of infants. TGF- was also detected at a higher frequency in response to AD IgG in TDP and TCD8 cells compared to mock and IVIG cultured conditions. An opposite effect was detected upon IFN- production in TCD4 cells, such that AD IgG reduced IFN- production compared to production under mock conditions but not under IVIG conditions. ConclusionIgG of AD patients can stimulate cytokine production in infant thymocytes and thus resembles the peripheral profile observed in adults. These findings suggest a novel mechanism that can contribute to AD pathogenesis. (AU)

• FAPESP's process: 15/17256-3 - "Mechanisms involved on offspring allergy development regulation by maternal immunization: role of cytokines, induction of T and B regulatory cells and allergen nature."
• Grantee: Alberto José da Silva Duarte
• Support Opportunities: Regular Research Grants