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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Holistic protocol for callus culture optimization using statistical modelling

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Author(s):
Fidemann, Tiago [1] ; de Araujo Pereira, Gabriela Aparecida [1] ; Nascimento, Lia Bossard [1] ; Moraes, Milena Cristina [1] ; Bertao, Monica Rosa [1] ; Goncalves da Silva, Regildo Marcio [1] ; Fernandez Nunez, Eutimio Gustavo [2]
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista UNESP, Dept Biotecnol, Fac Ciencias & Letras, Assis - Brazil
[2] Univ Fed ABC, CCNH, Santo Andre - Brazil
Total Affiliations: 2
Document type: Journal article
Source: NATURAL PRODUCT RESEARCH; v. 32, n. 9, p. 1109-1117, 2018.
Web of Science Citations: 0
Abstract

Plants endue a key role against illnesses caused by oxidative stress. These attributes are frequently associated with polyphenolic compounds. However, presence and concentration of secondary metabolites are affected by abiotic factors. The in vitro culture techniques can solve these drawbacks. Peppers can be a suitable alternative to obtain polyphenols. Aiming to optimise the callus culture stage from Capsicum baccatum to produce polyphenols, this work evaluated systemically the effects of the explant's origin (root, hypocotyl and cotyledon), growth hormone type (2,4-dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP) and a combination of 2,4-D/BAP at five-to-one ratio) and concentration (0.023-10.000mg L-1) on callus culture efficiency parameters using a multilevel factorial design. The root explant in combination with BAP at 1.138 mg L-1 ensured the optimal values of the assessed responses; callus mass (225.03 mg), antioxidant activity (35.95%), total phenols (11.48 mg of GAE/g DE) and flavonoids (15.92 mg of RU/g DE) production. {[}GRAPHICS] . (AU)

FAPESP's process: 14/26997-4 - Optimization of callus and suspension culture conditions for Capsicum baccatum var. pendulum cells
Grantee:Tiago Fidemann
Support Opportunities: Scholarships in Brazil - Master