Holistic protocol for callus culture optimization using statistical modelling

Author(s)

Fidemann, Tiago ^[1] ; de Araujo Pereira, Gabriela Aparecida ^[1] ; Nascimento, Lia Bossard ^[1] ; Moraes, Milena Cristina ^[1] ; Bertao, Monica Rosa ^[1] ; Goncalves da Silva, Regildo Marcio ^[1] ; Fernandez Nunez, Eutimio Gustavo ^[2]

Affiliation

^[1] Univ Estadual Paulista UNESP, Dept Biotecnol, Fac Ciencias & Letras, Assis - Brazil

^[2] Univ Fed ABC, CCNH, Santo Andre - Brazil

• Document type: Journal article
• Source: NATURAL PRODUCT RESEARCH; v. 32, n. 9, p. 1109-1117, 2018.
• Web of Science Citations: 0

Abstract

Plants endue a key role against illnesses caused by oxidative stress. These attributes are frequently associated with polyphenolic compounds. However, presence and concentration of secondary metabolites are affected by abiotic factors. The in vitro culture techniques can solve these drawbacks. Peppers can be a suitable alternative to obtain polyphenols. Aiming to optimise the callus culture stage from Capsicum baccatum to produce polyphenols, this work evaluated systemically the effects of the explant's origin (root, hypocotyl and cotyledon), growth hormone type (2,4-dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP) and a combination of 2,4-D/BAP at five-to-one ratio) and concentration (0.023-10.000mg L-1) on callus culture efficiency parameters using a multilevel factorial design. The root explant in combination with BAP at 1.138 mg L-1 ensured the optimal values of the assessed responses; callus mass (225.03 mg), antioxidant activity (35.95%), total phenols (11.48 mg of GAE/g DE) and flavonoids (15.92 mg of RU/g DE) production. {[}GRAPHICS] . (AU)

• FAPESP's process: 14/26997-4 - Optimization of callus and suspension culture conditions for Capsicum baccatum var. pendulum cells
• Grantee: Tiago Fidemann
• Support Opportunities: Scholarships in Brazil - Master