A novel research model for evaluating sunscreen protection in the UV-A1

Author(s)

Figueiredo, Sonia Aparecida ^[1] ; de Moraes, Dayane Cristina ^[1] ; Pinto Vilela, Fernanda Maria ^[2] ; de Faria, Amanda Natalina ^[3] ; dos Santos, Marcelo Henrique ^[4] ; Vieira Fonseca, Maria Jose ^[1]

Affiliation

^[1] Univ Sao Paulo, Qual Control & Photochemoprevent Lab, Fac Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Juiz de Fora, Fac Pharm, Rua Jose Lourenco Kelmer S-N, BR-36036900 Juiz De Fora, MG - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biochem, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Univ Fed Vicosa, Dept Chem, Ave Peter Henry Rolfs S-N, BR-36570000 Vicosa, MG - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 178, p. 61-68, JAN 2018.
• Web of Science Citations: 1

Abstract

The use of a broad spectrum sunscreen is considered one of the main and most popular measures for preventing the damaging effects of ultraviolet radiation (UVR) on the skin. In this study we have developed a novel in vitro method to assess sunscreens efficacy to protect calcineurin enzyme activity, a skin cell marker. The photoprotective efficacy of sunscreen products was assessed by measuring the UV-A1 radiation-induced depletion of calcineurin (Cn) enzyme activity in primary neonatal human dermal fibroblast (HDFn) cell lysates. After exposure to 24 J/cm(2) UV-A1 radiation, the sunscreens containing larger amounts of UV-A1 filters (brand B), the astaxanthin (UV-A1 absorber) and the Tinosorb (R) M (UV-A1 absorber) were capable of preventing loss of Cn activity when compared to the sunscreens formulations of brand A (low concentration of UV-A1 filters), with the Garcinia brasiliensis extract (UV-B absorber) and with the unprotected cell lysate and exposed to irradiation (Irradiated Control - IC). The Cn activity assay is a reproducible, accurate and selective technique for evaluating the effectiveness of sunscreens against the effects of UV-A1 radiation. The developed method showed that calcineurin activity have the potential to act as a biological indicator of UV-A1 radiation-induced damages in skin and the assay might be used to assess the efficacy of sunscreens agents and plant extracts prior to in vivo tests. (AU)

• FAPESP's process: 13/16311-5 - Development, standardization and validation of in vitro methods for assessing the efficacy of the photoprotective formulation in UVA-I, UVA-II and UVB bands
• Grantee: Maria José Vieira Fonseca
• Support Opportunities: Regular Research Grants