Ex vivo and in vivo establishment of new chemopreventive properties of UV filters

• Grant number: 19/16169-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2020
• End date: August 31, 2022
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: André Rolim Baby
• Grantee: André Rolim Baby
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo

Abstract

Although there are standard methods for evaluating photoprotection (UVA and UVB), the damage chemoprevention mechanisms caused by ultraviolet radiation are not considered in these methods. Urocanic acid is a chromophore found in the skin and has been identified as an important immunosuppression mediator and carcinogenesis through its photoisomerization the trans form to the cis form induced by ultraviolet radiation. The development of a new method that explores the isomerization investigation it is necessary to elucidated possible mechanisms of protection. Although the sunscreens use is an important measure against sun damage, the elucidation of their effects in chemoprevention it is necessary. This project aims to develop and apply an ex vivo, non-invasive tape stripping methodology for the urocanic acid isomers' determination in the epidermis and to evaluate the penetration effect of these filters, as well as the protection against photoisomerization of urocanic acid after irradiation of UV light. In addition, the same procedure will be used to determine the antioxidant activity (antilipoperoxidative) of these sunscreens, and thus it is expected to map and elucidate the effects of photobiological protection of sunscreens of different absorption on the stratum corneum. (AU)

Scientific publications

(25)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TÉRCIO ELYAN AZEVEDO MARTINS; CLAUDINÉIA APARECIDA SALES DE OLIVEIRA PINTO; ANDRESSA COSTA DE OLIVEIRA; FABIANA VIEIRA LIMA; MARIA VALÉRIA ROBLES VELASCO; LETÍCIA NORMA CARPENTIERI RODRIGUES; ANDRÉ ROLIM BABY. RP-HPLC simultaneous quantification of rutin, avobenzone, and octyl methoxycinnamate in the presence of hydroxypropyl β-cyclodextrin (HPβCD) and sulfobutyl ether β-cyclodextrin (SBEβCD). Brazilian Journal of Pharmaceutical Sciences, v. 58, 2023-01-23. (19/16169-0)

MOROCHO-JACOME, ANA LUCIA; FREIRE, THAMIRES BATELLO; DE OLIVEIRA, ANDRESSA COSTA; DE ALMEIDA, TANIA SANTOS; ROSADO, CATARINA; VELASCO, MARIA VALERIA ROBLES; BABY, ANDRE ROLIM. In vivo SPF from multifunctional sunscreen systems developed with natural compounds-A review. Journal of Cosmetic Dermatology, v. 20, n. 3, JUL 2020. (19/16169-0)

MUSSI, LILIAN; BABY, ANDRE ROLIM; CAMARGO JUNIOR, FLAVIO BUENO; PADOVANI, GIOVANA; SUFI, BIANCA DA SILVA; MAGALHAES, WAGNER VIDAL. Propanediol (and) Caprylic Acid (and) Xylitol as a New Single Topical Active Ingredient against Acne: In Vitro and In Vivo Efficacy Assays. Molecules, v. 26, n. 21, NOV 2021. (19/16169-0)

SAUCE, RAFAEL; SALES DE OLIVEIRA PINTO, CLAUDINEIA APARECIDA; AYALA-JARA, CARMEN; PRIETO, ZULITA ADRIANA; ROBLES VELASCO, MARIA VALERIA; BABY, ANDRE ROLIM. Preliminary Protocol Development of a HPLC-TBARS-EVSC (Ex Vivo Stratum Corneum) Assay for Skin Research: Application in a Sunscreen System. SCIENTIA PHARMACEUTICA, v. 89, n. 2, 2021. (16/24360-4, 19/16169-0)

FURUKAWA, JULIANA YUKA; MARTINEZ, RENATA MILIANI; MOROCHO-JACOME, ANA LUCIA; CASTILLO-GOMEZ, THALIA SELENE; PEREDA-CONTRERAS, VECXI JUDITH; ROSADO, CATARINA; VELASCO, MARIA VALERIA ROBLES; BABY, ANDRE ROLIM. Skin impacts from exposure to ultraviolet, visible, infrared, and artificial lights - a review. Journal of Cosmetic and Laser Therapy, v. 23, n. 1-2, JUL 2021. (19/16169-0)

HUBNER, ALEXANDRA ALMEIDA; DEMARQUE, DANIEL PECORARO; LOURENCO, FELIPE REBELLO; ROSADO, CATARINA; BABY, ANDRE ROLIM; KIKUCHI, IRENE SATIKO; BACCHI, ELFRIEDE MARIANNE. Phytocompounds Recovered from the Waste of Cabernet Sauvignon (Vitis vinifera L.) Vinification: Cytotoxicity (in Normal and Stressful Conditions) and In Vitro Photoprotection Efficacy in a Sunscreen System. COSMETICS, v. 10, n. 1, p. 10-pg., 2023-02-01. (19/16169-0)

CANDIDO, THALITA MARCILIO; ARIEDE, MAIRA BUENO; DE OLIVEIRA PINTO, CLAUDINEIA APARECIDA SALES; LIMA, FABIANA VIEIRA; MAGALHAES, WAGNER VIDAL; ESTEVES PEDRO, NATALIA MENCACCI; PADOVANI, GIOVANA; SUFI, BIANCA DA SILVA; RIJO, PATRICIA; ROBLES VELASCO, MARIA VALERIA; et al. Rosmarinic Acid Multifunctional Sunscreen: Comet Assay and In Vivo Establishment of Cutaneous Attributes. COSMETICS, v. 9, n. 6, p. 13-pg., 2022-12-01. (19/16169-0)

SARRUF, FERNANDA DAUD; ISSA, MICHELE GEORGES; VELASCO, MARIA VALERIA ROBLES; ROSADO, CATARINA; BABY, ANDRE ROLIM. Clay-Based Cosmetic Formulations: Mineralogical Properties and Short-Term Effects on Sebum Regulation and Skin Biomechanics. COSMETICS, v. 12, n. 5, p. 15-pg., 2025-10-04. (24/01920-0, 19/16169-0)

NERY, ERIKA MINUCI; MARTINEZ, RENATA MILIANI; VELASCO, MARIA VALERIA ROBLES; BABY, ANDRE ROLIM. A short review of alternative ingredients and technologies of inorganic UV filters. Journal of Cosmetic Dermatology, v. 20, n. 4, SEP 2020. (19/16169-0)

MARTINS, TERCIO ELYAN AZEVEDO; PINTO, CLAUDINEIA APARECIDA SALES DE OLIVEIRA; OLIVEIRA, ANDRESSA COSTA DE; LIMA, FABIANA VIEIRA; VELASCO, MARIA VALERIA ROBLES; RODRIGUES, LETICIA NORMA CARPENTIERI; BABY, ANDRE ROLIM. RP-HPLC simultaneous quantification of rutin, avobenzone, and octyl methoxycinnamate in the presence of hydroxypropyl ?-cyclodextrin (HP?CD) and sulfobutyl ether ?-cyclodextrin (SBE?CD). Brazilian Journal of Pharmaceutical Sciences, v. 58, p. 16-pg., 2022-01-01. (19/16169-0)

CANDIDO, THALITA MARCILIO; ARIEDE, MAIRA BUENO; LIMA, FABIANA VIEIRA; GUEDES, LUCIANA DE SOUZA; ROBLES VELASCO, MARIA VALERIA; BABY, ANDRE ROLIM; ROSADO, CATARINA. Dietary Supplements and the Skin: Focus on Photoprotection and Antioxidant Activity-A Review. NUTRIENTS, v. 14, n. 6, p. 15-pg., 2022-03-01. (19/16169-0)

CANDIDO, THALITA MARCILIO; ARIEDE, MAIRA BUENO; SALES DE OLIVEIRA PINTO, CLAUDINEIA APARECIDA; LOURENCO, FELIPE REBELLO; ROSADO, CATARINA; ROBLES VELASCO, MARIA VALERIA; BABY, ANDRE ROLIM. Prospecting In Vitro Antioxidant and Photoprotective Properties of Rosmarinic Acid in a Sunscreen System Developed by QbD Containing Octyl p-Methoxycinnamate and Bemotrizinol. COSMETICS, v. 9, n. 2, p. 11-pg., 2022-04-01. (19/16169-0)

LIMA, FABIANA VIEIRA; MARTINS, TERCIO ELYAN AZEVEDO; MOROCHO-JACOME, ANA LUCIA; ALMEIDA, ISABEL FILIPA; ROSADO, CATARINA FIALHO; VELASCO, MARIA VALERIA ROBLES; BABY, ANDRE ROLIM. Analytical tools for urocanic acid determination in human samples: A review. JOURNAL OF SEPARATION SCIENCE, NOV 2020. (19/16169-0)

LIMA, FABIANA VIEIRA; AZEVEDO MARTINS, TERCIO ELYAN; MOROCHO-JACOME, ANA LUCIA; ALMEIDA, ISABEL FILIPA; ROSADO, CATARINA FIALHO; ROBLES VELASCO, MARIA VALERIA; BABY, ANDRE ROLIM. Analytical tools for urocanic acid determination in human samples: A review. JOURNAL OF SEPARATION SCIENCE, v. 44, n. 1, SI, p. 438-447, JAN 2021. (19/16169-0)

MARTINEZ, RENATA MILIANI; OSELIERO FILHO, PEDRO LEONIDAS; GERBELLI, BARBARA BIANCA; MAGALHAES, WAGNER VIDAL; VELASCO, MARIA VALERIA ROBLES; DA SILVA LANNES, SUZANA CAETANO; DE OLIVEIRA, CRISTIANO LUIS PINTO; ROSADO, CATARINA; BABY, ANDRE ROLIM. Influence of the Mixtures of Vegetable Oil and Vitamin E over the Microstructure and Rheology of Organogels. GELS, v. 8, n. 1, JAN 2022. (16/24531-3, 20/02192-8, 19/16169-0, 19/12301-1, 18/05888-3, 20/13204-7)

MACHADO, GUSTAVO TEIXEIRA; CHIABAI, CAIO RUI; PINHEIRO, MARIANA SANTOS; PINTO, CLAUDINEIA APARECIDA SALES DE OLIVEIRA; BABY, ANDRE ROLIM; ANDRADE, GEORGE RICARDO SANTANA; PESSOA, FABIANA VIEIRA LIMA SOLINO. In vitro photoprotective efficacy and photostability of synthesized star-shaped ZnO nanoaggregates associated with ethylhexyl methoxycinnamate and butyl methoxydibenzoylmethane. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 261, p. 8-pg., 2024-11-29. (19/16169-0, 24/01920-0)

RUSCINC, NADIA; SERAFIM, RICARDO AUGUSTO MASSARICO; ALMEIDA, CINTIA; ROSADO, CATARINA; BABY, ANDRE ROLIM. Challenging the safety and efficacy of topically applied chlorogenic acid, apigenin, kaempferol, and naringenin by HET-CAM, HPLC-TBARS-EVSC, and laser Doppler flowmetry. RONTIERS IN CHEMISTR, v. 12, p. 11-pg., 2024-05-20. (19/16169-0)

SAUCE, RAFAEL; SALES DE OLIVEIRA PINTO, CLAUDINEIA APARECIDA; ROBLES VELASCO, MARIA VALERIA; ROSADO, CATARINA; BABY, ANDRE ROLIM. Ex vivo penetration analysis and anti-inflammatory efficacy of the association of ferulic acid and UV filters. European Journal of Pharmaceutical Sciences, v. 156, JAN 1 2021. (19/16169-0, 16/24360-4)

BABY, ANDRE ROLIM; FREIRE, THAMIRES BATELLO; MARQUES, GABRIELA DE ARGOLLO; RIJO, PATRICIA; LIMA, FABIANA VIEIRA; MONTEIRO DE CARVALHO, JOAO CARLOS; ROJAS, JOHN; MAGALHAES, WAGNER VIDAL; ROBLES VELASCO, MARIA VALERIA; MOROCHO-JACOME, ANA LUCIA. Azadirachta indica (Neem) as a Potential Natural Active for Dermocosmetic and Topical Products: A Narrative Review. COSMETICS, v. 9, n. 3, p. 17-pg., 2022-06-01. (19/16169-0)

DE CASTRO, KARINE CAPPUCCIO; COCO, JULIA CEDRAN; DOS SANTOS, ERICA MENDES; ATAIDE, JANAINA ARTEM; MARTINEZ, RENATA MILIANI; MONTEIRO DO NASCIMENTO, MONICA HELENA; PRATA, JOAO; LOPES DA FONTE, PEDRO RICARDO MARTINS; SEVERINO, PATRICIA; MAZZOLA, PRISCILA GAVA; et al. Pluronic (R) triblock copolymer-based nanoformulations for cancer therapy: A 10-year overview. JOURNAL OF CONTROLLED RELEASE, v. 353, p. 21-pg., 2022-12-17. (18/10799-0, 20/03727-2, 21/00319-3, 19/20303-4, 20/11333-4, 18/06475-4, 19/16169-0, 18/07707-6, 17/10789-1, 19/08549-8)

LIMA, FABIANA VIEIRA; AZEVEDO MARTINS, TERCIO ELYAN; MOROCHO-JACOME, ANA LUCIA; ALMEIDA, ISABEL FILIPA; ROSADO, CATARINA FIALHO; ROBLES VELASCO, MARIA VALERIA; BABY, ANDRE ROLIM. Analytical tools for urocanic acid determination in human samples: A review. JOURNAL OF SEPARATION SCIENCE, v. 44, n. 1, p. 10-pg., 2020-11-03. (19/16169-0)

DE SOUZA GUEDES, LUCIANA; MARTINEZ, RENATA MILIANI; BOU-CHACRA, NADIA A.; VELASCO, MARIA VALERIA ROBLES; ROSADO, CATARINA; BABY, ANDRE ROLIM. An Overview on Topical Administration of Carotenoids and Coenzyme Q10 Loaded in Lipid Nanoparticles. ANTIOXIDANTS, v. 10, n. 7, JUL 2021. (19/16169-0)

RUSCINC, NADIA; MOROCHO-JACOME, ANA LUCIA; MARTINEZ, RENATA MILIANI; MAGALHAES, WAGNER VIDAL; ESCUDEIRO, CASSIANO CARLOS; GIAROLLA, JEANINE; ROSADO, CATARINA; ROBLES VELASCO, MARIA VALERIA; BABY, ANDRE ROLIM. Vaccinium myrtillus L. extract associated with octocrylene, bisoctrizole, and titanium dioxide: in vitro and in vivo tests to evaluate safety and efficacy. Journal of Cosmetic Dermatology, v. N/A, p. 10-pg., 2022-01-23. (19/16169-0)

MOROCHO-JACOME, ANA LUCIA; SANTOS, BRUNA BERTOLONI DOS; CARVALHO, JOAO CARLOS MONTEIRO DE; ALMEIDA, TANIA SANTOS DE; RIJO, PATRICIA; VELASCO, MARIA VALERIA ROBLES; ROSADO, CATARINA; BABY, ANDRE ROLIM. Microalgae as a Sustainable, Natural-Oriented and Vegan Dermocosmetic Bioactive Ingredient: The Case of Neochloris oleoabundans. COSMETICS, v. 9, n. 1, p. 11-pg., 2022-02-01. (19/16169-0, 15/11194-6, 16/22000-0)

MARQUES, GABRIELA DE ARGOLLO; HIRAISHI, CAMILA FAUSTINO; MACEDO, PEDRO IVO DE SOUZA; PINTO, CLAUDINEIA APARECIDA SALES DE OLIVEIRA; GREGORIO, JOAO; ROSADO, CATARINA; VELASCO, MARIA VALERIA ROBLES; BABY, ANDRE ROLIM. HPLC-TBARS-EVSC (high-performance liquid chromatography-thiobarbituric acid reactive substances-ex vivo stratum corneum) protocol: Selection of the subjects and approach to present the results. International Journal of Cosmetic Science, v. 45, n. 5, p. 8-pg., 2023-10-01. (19/16169-0, 21/13276-0)