Effects of local administration of tiludronic acid on experimental periodontitis in diabetic rats

Author(s)

Nunes, Nara L. T. ^[1] ; Messora, Michel R. ^[2] ; Oliveira, Luiz Fernando ^[2] ; Lisboa, Mario ^[3] ; Barcellos Garcia, Marina Costa ^[2] ; Rego, R. O. ^{[4, 5]} ; Novaes, Jr., Arthur Belem ^[2] ; Scombatti de Souza, Sergio Luis ^[2] ; Ervolino, Edilson ^[5] ; Furlaneto, Flavia A. C. ^[2]

Affiliation

^[1] Univ Fed Ceara, Dept Clin Dent, Grad Program Dent, Fac Pharm Dent & Nursing, Fortaleza, Ceara - Brazil

^[2] Univ Sao Paulo, Dept Oral & Maxillofacial Surg & Periodontol, Sch Dent Ribeirao Preto, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Ceara, Fac Med, Dept Morphol, Sobral, Ceara - Brazil

^[4] Univ Fed Ceara, Dept Dent, Sch Dent Sobral, Sobral, Ceara - Brazil

^[5] Sao Paulo State Univ, Dent Sch Aracatuba, Div Histol, Dept Basic Sci, Aracatuba, SP - Brazil

• Document type: Journal article
• Source: Journal of Periodontology; v. 89, n. 1, p. 105-116, JAN 2018.
• Web of Science Citations: 0

Abstract

Background: Tiludronic acid (TIL) presents antiresorptive and anti-inflammatory properties and has not been evaluated in the periodontitis-diabetes mellitus (DM) association to date, to the best knowledge of the authors. This study evaluates effects of local administration of TIL on experimental periodontitis (EP) in rats with streptozotocin-induced DM. Methods: Thirty-two animals (Rattus norvegicus albinus, Wistar) were divided into groups DM/C (Control), DM/EP, DM/EP/TIL1, and DM/EP/TIL3. In EP groups, a ligature was placed around mandibular first molars. In groups DM/EP/TIL1 and DM/EP/TIL3, TIL solutions (1 and 3 mg/kg, respectively) were injected into the gingival tissue of mandibular molars every other day for 10 days, until euthanasia. Periodontal tissues were analyzed by microcomputed tomography (micro-CT), histomorphometry, immunohistochemistry (tartrate-resistant acid phosphatase {[}TRAP], receptor activator of nuclear factor-kappa B ligand {[}RANKL], osteoprotegerin, cleaved caspase 3), and quantitative reverse transcription-polymerase chain reaction (interleukin {[}IL]-1 beta,vascular endothelial growth factor {[}VEGF]). Results: In micro-CT analyses, groups DM/EP/TIL1 and DM/EP/TIL3 presented reduced alveolar bone resorption (P < 0.05). Group DM/EP/TIL3 presented decreased attachment loss (P < 0.05). The amount of TRAP-positive multinucleated cells was decreased in TIL groups (P < 0.05). Group DM/EP/TIL3 presented a lower immunolabeling pattern for RANKL (P < 0.05). TIL treatment decreased genic expression of IL-1 beta, and in group DM/EP/TIL3, expression of VEGF was increased (P < 0.05). Conclusion: Local administration of TIL promoted a protective effect against tissue destruction in EP in diabetic rats, and the dosage of 3 mg/kg of TIL promoted the best results regarding its antiresorptive and anti-inflammatory effects. (AU)

• FAPESP's process: 15/23701-0 - Evaluation of the effects of local administration of tiludronic acid on experimental periodontitis in diabetic rat: a immunohistochemical analysis
• Grantee: Marina Costa Barcellos Garcia
• Support Opportunities: Scholarships in Brazil - Scientific Initiation